Typical Testing Data/Standard Curve (for reference only)
Mouse Vanin 3 (VNN3) ELISA Kit | VNN3 elisa kit
Mouse Vanin 3 (VNN3) ELISA Kit
Gene Names
VNN3; HSA238982
Reactivity
Mouse
Synonyms
Vanin 3 (VNN3); Mouse Vanin 3 (VNN3) ELISA Kit; Vascular non-inflammatory molecule 3; VNN3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of VNN3. No significant cross-reactivity or interference between VNN3 and analogues was observed.
Sequence Length
117
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.114ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VNN3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VNN3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VNN3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of VNN3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VNN3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VNN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VNN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VNN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VNN3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VNN3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VNN3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VNN3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,201 Da
NCBI Official Full Name
Vanin 3
NCBI Official Synonym Full Names
vanin 3
NCBI Official Symbol
VNN3
NCBI Official Synonym Symbols
HSA238982
NCBI Protein Information
vascular non-inflammatory molecule 3
UniProt Protein Name
Vascular non-inflammatory molecule 3
Protein Family
UniProt Gene Name
VNN3
UniProt Synonym Gene Names
Vanin-3
NCBI Description
This gene is the central gene in a cluster of three vanin genes on chromosome 6q23-q24. Extensive alternative splicing has been described; the two most common variants are represented as RefSeqs. [provided by RefSeq, Apr 2014]
Uniprot Description
VNN3: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. Belongs to the CN hydrolase family. BTD/VNN subfamily. Induced by Th17/Th1 type cytokines, but not by Th2-type. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.1.92; Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 6q23.2
Cellular Component: extracellular region; plasma membrane
Molecular Function: pantetheine hydrolase activity
Biological Process: C-terminal protein lipidation; pantothenate metabolic process
Research Articles on VNN3
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Product Notes
The Mouse VNN3 vnn3 (Catalog #AAA2705278) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2705278 ELISA Kit recognizes Mouse VNN3. It is sometimes possible for the material contained within the vial of "Vanin 3 (VNN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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