Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Human Lactoferrin (LTF) ELISA Kit | LTF elisa kit
Human Lactoferrin (LTF) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Lactoferrin (LTF) Standard Curve.)
Background: LTF, a globular multifunctional protein with antimicrobial activity (bacteriocide, fungicide), is part of the innate defense, mainly at mucoses. Lactoferrin is found in milk and many mucosal secretions such as tears and saliva. Lactoferrin is also present in secondary granules of PMN and also is secreted by some acinar cells. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin belongs to the transferrin family proteins (TF, melanotransferrin, ovotransferin, etc.). Its molecular mass is 80,000 u (80 kDa).Lactoferrin antimicrobial activity is due partly to its high affinity for Fe (ferric state). LF proteolysis produces lactoferricin, kaliocin-1 small peptides with antimicrobial activity.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Antimicrobial, antiviral, antifungal and antiparasitic activity has been found for this protein and its peptides. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
Uniprot Description
LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.
Protein type: EC 3.4.21.-; Secreted; Protease; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3p21.31
Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule
Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; ferric iron binding; DNA binding; serine-type endopeptidase activity; iron ion binding
Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; interaction with host; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; regulation of cytokine production; activation of NF-kappaB transcription factor; retinal homeostasis; positive regulation of osteoblast differentiation; innate immune response in mucosa; negative regulation of viral genome replication; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of ATPase activity; negative regulation of apoptosis
Research Articles on LTF
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Product Notes
The Human LTF ltf (Catalog #AAA9715430) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715430 ELISA Kit recognizes Human LTF. It is sometimes possible for the material contained within the vial of "Lactoferrin (LTF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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