Human Kv channel-interacting protein 4 (KCNIP4) ELISA Kit | KCNIP4 elisa kit

Human Kv channel-interacting protein 4 (KCNIP4) ELISA Kit

Cat. Num. AAA167172

• Gene Names: KCNIP4; CALP; KCHIP4
• Reactivity: Human
• Synonyms: Kv channel-interacting protein 4 (KCNIP4); Human Kv channel-interacting protein 4 (KCNIP4) ELISA Kit; KCNIP4 elisa kit

• Reactivity: Human
• Sequence Length: 229

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 30ng/ml
• Sensitivity: 0.024ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KCNIP4 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Kv Channel-interacting Protein 4 (also known as KCNIP4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human KCNIP4 antibody. KCNIP4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human KCNIP4 Antibody is added and binds to KCNIP4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNIP4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human KCNIP4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 22212902
• NCBI GeneID: 80333
• NCBI Accession #: NP_671712.1
• NCBI GenBank Nucleotide #: NM_147183.3
• UniProt Accession #: Q6PIL6; Q3YAB8; Q3YAB9; Q3YAC0; Q3YAC1; Q3YAC2; Q4W5G8; Q8NEU0; Q9BWT2; Q9H294; Q9H2A4
• Molecular Weight: 25,008 Da
• NCBI Official Full Name: Kv channel-interacting protein 4 isoform 4
• NCBI Official Synonym Full Names: potassium voltage-gated channel interacting protein 4
• NCBI Official Symbol: KCNIP4
• NCBI Official Synonym Symbols: CALP; KCHIP4
• NCBI Protein Information: Kv channel-interacting protein 4
• UniProt Protein Name: Kv channel-interacting protein 4
• Protein Family: Kv channel-interacting protein
• UniProt Gene Name: KCNIP4
• UniProt Synonym Gene Names: CALP; KCHIP4; KChIP4

NCBI Description

This gene encodes a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. This protein member also interacts with presenilin. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates KCND2 channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:11847232, PubMed:18957440, PubMed:23576435). Modulates KCND3/Kv4.3 currents (PubMed:23576435). Isoform 4 does not increase KCND2 expression at the cell membrane (PubMed:18957440). Isoform 4 retains KCND3 in the endoplasmic reticulum and negatively regulates its expression at the cell membrane.