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Human KRIT1, ankyrin repeat containing ELISA Kit | KRIT1 elisa kit

Human Krev interaction trapped protein 1, KRIT1 ELISA Kit

Gene Names
KRIT1; CAM; CCM1
Reactivity
Human
Synonyms
KRIT1; ankyrin repeat containing; Human Krev interaction trapped protein 1; KRIT1 ELISA Kit; Human Krev interaction trapped protein 1 (KRIT1) ELISA kit; CAM; CCM1; ankyrin repeat-containing protein Krit1; krev interaction trapped 1; KRIT1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
No significant cross-reactivity or interference between this analyte and analogues is observed.
Samples
Body fluids, tissue homogenates, secretions or feces samples
Assay Type
Quantitative Sandwich
Detection Range
0.25ng/ml-8ng/ml
Sensitivity
0.1ng/ml.
Intra-assay Precision
Intra-assay CV (%) is less than 15%
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for KRIT1 elisa kit
Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of KRIT1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
889
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
84,348 Da
NCBI Official Full Name
krev interaction trapped protein 1 isoform 2
NCBI Official Synonym Full Names
KRIT1, ankyrin repeat containing
NCBI Official Symbol
KRIT1
NCBI Official Synonym Symbols
CAM; CCM1
NCBI Protein Information
krev interaction trapped protein 1; krev interaction trapped 1; ankyrin repeat-containing protein Krit1; cerebral cavernous malformations 1 protein
UniProt Protein Name
Krev interaction trapped protein 1
UniProt Gene Name
KRIT1
UniProt Synonym Gene Names
CCM1; Krev interaction trapped 1
UniProt Entry Name
KRIT1_HUMAN

NCBI Description

This gene encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

KRIT1: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits EKR1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels. Defects in KRIT1 are the cause of cerebral cavernous malformations type 1 (CCM1). Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 7q21.2

Cellular Component: extracellular space; microtubule; cytoplasm; plasma membrane; intercellular junction

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; microtubule binding; protein complex binding

Biological Process: negative regulation of angiogenesis; positive regulation of protein binding; regulation of catalytic activity; cell redox homeostasis; small GTPase mediated signal transduction; negative regulation of endothelial cell proliferation; angiogenesis

Disease: Cerebral Cavernous Malformations

Research Articles on KRIT1

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Product Notes

The Human KRIT1 krit1 (Catalog #AAA9339178) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9339178 ELISA Kit recognizes Human KRIT1. It is sometimes possible for the material contained within the vial of "KRIT1, ankyrin repeat containing, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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