Mouse APG5L/ATG5 Monoclonal Antibody | anti-APG5L/ATG5 antibody

APG5L/ATG5 (3C10) Mouse mAb

Cat. Num. AAA4753876

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: APG5L/ATG5; Monoclonal Antibody; APG5L/ATG5 (3C10) Mouse mAb; APG5; APG5L; ASP; Autophagy protein 5; anti-APG5L/ATG5 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3C10-5C8-8C3
• Specificity: The ATG5 mouse monoclonal Antibody can detects endogenous ATG5 proteins.
• Purity/Purification: Affinity purified
• Form/Format: PBS(pH7.4) containing with 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-APG5L/ATG5 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:5;000-10;000; IHC: 1:100-200
• Immunogen: Purified recombinant protein expressed in E Coli.
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Cytoplasm, Membrane
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 Mouse mAb diluted at 1:200.)

Western Blot (WB)

(Western blot detection of Atg5/Apg5L (3C10) in C2C12,K562,C6,Mouse brain cell lysates using Atg5/Apg5L (3C10) antibody. Observed band size:55kDa.)

Related Product Information for anti-APG5L/ATG5 antibody

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

Product Categories/Family for anti-APG5L/ATG5 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 17366828
• NCBI GeneID: 9474
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7
• Molecular Weight: 275
• NCBI Official Full Name: Autophagy protein 5
• NCBI Official Synonym Full Names: autophagy related 5
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; apoptosis-specific protein; ATG5 autophagy related 5 homolog
• UniProt Protein Name: Autophagy protein 5
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; autophagic vacuole formation; negative regulation of apoptosis