Human Kinesin-Like Protein KIF1A (KIF1A) ELISA Kit | KIF1A elisa kit

Human Kinesin-Like Protein KIF1A (KIF1A) ELISA Kit

Cat. Num. AAA9715582

• Gene Names: KIF1A; ATSV; MRD9; HSN2C; SPG30; UNC104; C2orf20
• Reactivity: Human
• Synonyms: Kinesin-Like Protein KIF1A (KIF1A); Human Kinesin-Like Protein KIF1A (KIF1A) ELISA Kit; KIF1A; ATSV; C2orf20; DKFZp686I2094; FLJ30229; HUNC-104; MGC133285; MGC133286; UNC104; axonal transport of synaptic vesicles; kinesin; heavy chain; member 1A; homolog of mouse; tmp_locus_27; KIF1A elisa kit

• Reactivity: Human
• Sequence Length: 1689

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KIF1A elisa kit

Principle of the Assay: Human Kinesin-Like Protein KIF1A (KIF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF1A in samples. An antibody specific for KIF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KIF1A present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human KIF1A detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of KIF1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: KIF1A is a member of the kinesin family. This protein is highly similar to mouse heavy chain kinesin member 1A protein which is an anterograde motor protein that transports membranous organelles along axonal microtubules. It is thought that this protein may play a critical role in the development of axonal neuropathies resulting from impaired axonal transport. There are multiple polyadenylation sites found in this gene. This switch-like transition for movement could be shifted to lower PtdIns(4,5)P2 concentrations by the addition of cholesterol/sphingomyelin or GM1 ganglioside/cholera toxin, conditions that produced raft-like behavior of Unc104 bound to lipid bilayers.

NCBI and Uniprot Product Information

• NCBI GI #: 119591621
• NCBI GeneID: 547
• UniProt Accession #: Q12756
• NCBI Official Full Name: kinesin family member 1A, isoform CRA_b
• NCBI Official Synonym Full Names: kinesin family member 1A
• NCBI Official Symbol: KIF1A
• NCBI Official Synonym Symbols: ATSV; MRD9; HSN2C; SPG30; UNC104; C2orf20
• NCBI Protein Information: kinesin-like protein KIF1A
• UniProt Protein Name: Kinesin-like protein KIF1A
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF1A
• UniProt Synonym Gene Names: ATSV; C2orf20; hUnc-104
• UniProt Entry Name: KIF1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the kinesin family and functions as an anterograde motor protein that transports membranous organelles along axonal microtubules. Mutations at this locus have been associated with spastic paraplegia-30 and hereditary sensory neuropathy IIC. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012]

Uniprot Description

KIF1A: a member of the kinesin motor protein family. Motor for anterograde axonal transport of synaptic vesicle precursors. Interacts with PPFIA1 and PPFIA4. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: kinesin complex; microtubule; neuron projection; cell soma; cytoplasm

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: vesicle-mediated transport; metabolic process; cytoskeleton-dependent intracellular transport; anterograde axon cargo transport; microtubule-based movement

Disease: Neuropathy, Hereditary Sensory, Type Iic; Spastic Paraplegia 30, Autosomal Recessive; Neuropathy, Hereditary Sensory And Autonomic, Type Iia; Mental Retardation, Autosomal Dominant 9