Thyrotroph Embryonic Factor (TEF) CLIA Kit | TEF clia kit

CLIA Kit for Thyrotroph Embryonic Factor (TEF)

Cat. Num. AAA2000404

• Synonyms: Thyrotroph Embryonic Factor (TEF); CLIA Kit for Thyrotroph Embryonic Factor (TEF); TEF clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Thyrotroph Embryonic Factor (TEF); No significant cross-reactivity or interference between Thyrotroph Embryonic Factor (TEF) and analogues was observed.
• Sequence Length: 273

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 6.859-5000pg/mL The standard curve concentrations used for the ELISA's were 5000pg/mL, 1666.67pg/mL, 555.56pg/mL, 185.19pg/mL, 61.728pg/mL, 20.576pg/mL, 6.859pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 32pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyrotroph Embryonic Factor (TEF) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyrotroph Embryonic Factor (TEF) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Mus musculus (Mouse)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TEF clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thyrotroph Embryonic Factor (TEF) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 223972670
• NCBI GeneID: 7008
• NCBI Accession #: NP_001138870.1
• NCBI GenBank Nucleotide #: NM_001145398.2
• UniProt Accession #: Q10587; Q15729; Q7Z3J7; Q8IU94; Q96TG4; B0QYS8; B2RC22
• Molecular Weight: 33,248 Da
• NCBI Official Full Name: thyrotroph embryonic factor isoform 2
• NCBI Official Synonym Full Names: thyrotrophic embryonic factor
• NCBI Official Symbol: TEF
• NCBI Protein Information: thyrotroph embryonic factor
• UniProt Protein Name: Thyrotroph embryonic factor
• Protein Family: Transcription factor
• UniProt Gene Name: TEF
• UniProt Synonym Gene Names: KIAA1655
• UniProt Entry Name: TEF_HUMAN

NCBI Description

This gene encodes a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

TEF: Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'- [TC][AG][AG]TTA[TC][AG]-3'. Belongs to the bZIP family. PAR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: nucleus

Molecular Function: protein homodimerization activity; sequence-specific DNA binding; protein heterodimerization activity; double-stranded DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter