Human Kinesin Family, Member 5A (KIF5A) ELISA Kit | KIF5A elisa kit

Human Kinesin Family, Member 5A (KIF5A) ELISA Kit

Cat. Num. AAA2705827

• Gene Names: KIF5A; NKHC; MY050; NEIMY; SPG10; D12S1889
• Reactivity: Human
• Synonyms: Kinesin Family; Member 5A (KIF5A); Human Kinesin Family; Member 5A (KIF5A) ELISA Kit; NKHC; MY050; SPG10; Spastic Paraplegia 10(Autosomal Dominant); Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; KIF5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kinesin Family, Member 5A (KIF5A).; No significant cross-reactivity or interference between Kinesin Family, Member 5A (KIF5A) and analogues was observed.
• Sequence Length: 1032

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin Family, Member 5A (KIF5A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin Family, Member 5A (KIF5A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KIF5A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5A (KIF5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 5A (KIF5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5A (KIF5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 5A (KIF5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119617436
• NCBI GeneID: 3798
• Molecular Weight: 117,378 Da
• NCBI Official Full Name: kinesin family member 5A
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: KIF5A
• NCBI Official Synonym Symbols: NKHC; MY050; NEIMY; SPG10; D12S1889
• NCBI Protein Information: kinesin heavy chain isoform 5A
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• Protein Family: Kinesin
• UniProt Gene Name: KIF5A
• UniProt Synonym Gene Names: NKHC1; NKHC

NCBI Description

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: cell soma; ciliary rootlet; cytosol; dendrite cytoplasm; kinesin complex; membrane; microtubule; perinuclear region of cytoplasm

Molecular Function: ATP binding; ATP-dependent microtubule motor activity, plus-end-directed; kinesin binding; microtubule binding; motor activity; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; axon guidance; cytoskeleton-dependent intracellular transport; microtubule-based movement; protein localization; retrograde vesicle-mediated transport, Golgi to ER; synaptic transmission; vesicle-mediated transport

Disease: Myoclonus, Intractable, Neonatal; Spastic Paraplegia 10, Autosomal Dominant