Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 5 (KRT5) Standard Curve.)
Human Keratin, Type II Cytoskeletal 5 (KRT5) ELISA Kit | KRT5 elisa kit
Human Keratin, Type II Cytoskeletal 5 (KRT5) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 5 (KRT5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type II cytoskeletal 5 (KRT5) Standard Curve.)
Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic (i.e., different types of) keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. The type II cytokeratin genes are clustered in a region of chromosome 12q12-q13. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Eckert and Rorke (1988) cloned full-length KRT5 from a cultured keratinocyte cDNA library. The deduced 590-amino acid protein contains a central alpha-helical core region of about 310 amino acids with a 7-amino acid repeat found in coiled-coil structures. The N and C termini are serine rich and contain gly-gly-gly-x motifs.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Uniprot Description
K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 12q13.13
Cellular Component: membrane; mitochondrion; keratin filament; cytoplasm; plasma membrane; intermediate filament; cytosol; nucleus
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: hemidesmosome assembly; epidermis development
Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type
Research Articles on KRT5
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Product Notes
The Human KRT5 krt5 (Catalog #AAA9715540) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715540 ELISA Kit recognizes Human KRT5. It is sometimes possible for the material contained within the vial of "Keratin, Type II Cytoskeletal 5 (KRT5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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