Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 9 (KRT9) Standard Curve.)
Human Keratin, Type I Cytoskeletal 9 (KRT9) ELISA Kit | KRT9 elisa kit
Human Keratin, Type I Cytoskeletal 9 (KRT9) ELISA Kit
Gene Names
KRT9; K9; CK-9; EPPK
Reactivity
Human
Synonyms
Keratin; Type I Cytoskeletal 9 (KRT9); Human Keratin; Type I Cytoskeletal 9 (KRT9) ELISA Kit; KRT9; CK-9; EPPK; K9; cytokeratin 9; type I cytoskeletal 9; KRT9 elisa kit
Reactivity
Human
Specificity
Human Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit has high sensitivity and excellent specificity for detection of Human KRT9. No significant cross-reactivity or interference between Human KRT9 and analogues was observed.
Sequence Length
623
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 9 (KRT9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 9 (KRT9) Standard Curve.)
Related Product Information for KRT9 elisa kit
Description: This Human Keratin, type I cytoskeletal 9 (KRT9) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT9 in samples. An antibody specific for KRT9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Keratin 9 is a type I cytokeratin. It is found only in the terminally differentiated epidermis of palms and soles. Mutations in the gene encoding this protein cause epidermolytic palmoplantar keratoderma.Langbein et al. (1993) reported the cloning and sequence of the KRT9 gene. The amino acid sequence showed the typical structure of type I cytokeratins, with a head (153 residues), alpha-helical coiled-coil-forming rod (306 residues), and tail (163 residues) domains. The keratin 9 protein displayed highest homology to human keratin 10, not only in the highly conserved rod domain but also in large parts of the head and tail domains. Expression was confined to palmar and plantar epidermis. In situ hybridization and immunolocalization showed expression in subrabasal cell layers.
Background: Keratin 9 is a type I cytokeratin. It is found only in the terminally differentiated epidermis of palms and soles. Mutations in the gene encoding this protein cause epidermolytic palmoplantar keratoderma.Langbein et al. (1993) reported the cloning and sequence of the KRT9 gene. The amino acid sequence showed the typical structure of type I cytokeratins, with a head (153 residues), alpha-helical coiled-coil-forming rod (306 residues), and tail (163 residues) domains. The keratin 9 protein displayed highest homology to human keratin 10, not only in the highly conserved rod domain but also in large parts of the head and tail domains. Expression was confined to palmar and plantar epidermis. In situ hybridization and immunolocalization showed expression in subrabasal cell layers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type I cytoskeletal 9
NCBI Official Synonym Full Names
keratin 9
NCBI Official Symbol
KRT9
NCBI Official Synonym Symbols
K9; CK-9; EPPK
NCBI Protein Information
keratin, type I cytoskeletal 9
UniProt Protein Name
Keratin, type I cytoskeletal 9
Protein Family
UniProt Gene Name
KRT9
UniProt Synonym Gene Names
CK-9; K9
NCBI Description
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008]
Uniprot Description
May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly.
Research Articles on KRT9
Similar Products
Product Notes
The Human KRT9 krt9 (Catalog #AAA9716279) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716279 ELISA Kit recognizes Human KRT9. It is sometimes possible for the material contained within the vial of "Keratin, Type I Cytoskeletal 9 (KRT9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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