Human Karyopherin Alpha 2 (KPNa2) ELISA Kit | KPNa2 elisa kit

Human Karyopherin Alpha 2 (KPNa2) ELISA Kit

Cat. Num. AAA2019577

• Gene Names: KPNA2; QIP2; RCH1; IPOA1; SRP1alpha
• Reactivity: Human
• Synonyms: Karyopherin Alpha 2 (KPNa2); Human Karyopherin Alpha 2 (KPNa2) ELISA Kit; IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha; KPNa2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Karyopherin Alpha 2 (KPNa2).; No significant cross-reactivity or interference between Karyopherin Alpha 2 (KPNa2) and analogues was observed.
• Sequence Length: 529

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 125-8000pg/mL
• Sensitivity: < 52pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Karyopherin Alpha 2 (KPNa2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KPNa2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Alpha 2 (KPNa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Karyopherin Alpha 2 (KPNa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Karyopherin Alpha 2 (KPNa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Karyopherin Alpha 2 (KPNa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for KPNa2 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 15865453
• NCBI GeneID: 3838
• UniProt Accession #: P52292; Q53YE3; Q9BRU5; B9EJD6
• Molecular Weight: 57,862 Da
• NCBI Official Full Name: karyopherin alpha 2
• NCBI Official Synonym Full Names: karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
• NCBI Official Symbol: KPNA2
• NCBI Official Synonym Symbols: QIP2; RCH1; IPOA1; SRP1alpha
• NCBI Protein Information: importin subunit alpha-1
• UniProt Protein Name: Importin subunit alpha-1
• UniProt Gene Name: KPNA2
• UniProt Synonym Gene Names: RCH1; SRP1
• UniProt Entry Name: IMA1_HUMAN

NCBI Description

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA2: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.

Protein type: Karyopherin; Nucleoporin; Nuclear import

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol

Molecular Function: protein binding; histone deacetylase binding; nuclear localization sequence binding; protein transporter activity

Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; regulation of DNA recombination; NLS-bearing substrate import into nucleus; DNA metabolic process