Immunofluorescence (IF)
(Fluorescent image of MCF-7 cells stained with ZNF434 Antibody (Center). MBS9212596 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit anti-Human ZNF434 Polyclonal Antibody | anti-ZSCAN32 antibody
ZNF434 Antibody (Center)
Gene Names
ZSCAN32; HCCS-5; ZNF434
Reactivity
Human
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ZNF434; Polyclonal Antibody; ZNF434 Antibody (Center); Zinc finger and SCAN domain-containing protein 32; Human cervical cancer suppressor gene 5 protein; HCCS-5; Zinc finger protein 434; ZSCAN32; anti-ZSCAN32 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ZNF434 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-187 amino acids from the Central region of human ZNF434.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
159-187
Sequence Length
408
Applicable Applications for anti-ZSCAN32 antibody
Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)
Application Notes
IF~~1:25
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cells stained with ZNF434 Antibody (Center). MBS9212596 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of MCF-7 cells stained with ZNF434 Antibody (Center). MBS9212596 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(ZNF434 Antibody (Center) western blot analysis in Jurkat,MCF-7 cell line lysates (35ug/lane).This demonstrates the ZNF434 antibody detected the ZNF434 protein (arrow).)
Western Blot (WB)
(ZNF434 Antibody (Center) western blot analysis in Jurkat,MCF-7 cell line lysates (35ug/lane).This demonstrates the ZNF434 antibody detected the ZNF434 protein (arrow).)
Related Product Information for anti-ZSCAN32 antibody
May be involved in transcriptional regulation.
Product Categories/Family for anti-ZSCAN32 antibody
References
Shan Y.X., et al. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
Kim J.W., et al. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).
Kim J.W., et al. Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
Ota T., et al. Nat. Genet. 36:40-45(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
78728
NCBI Official Full Name
zinc finger and SCAN domain-containing protein 32 isoform b
NCBI Official Synonym Full Names
zinc finger and SCAN domain containing 32
NCBI Official Symbol
ZSCAN32
NCBI Official Synonym Symbols
HCCS-5; ZNF434
NCBI Protein Information
zinc finger and SCAN domain-containing protein 32
UniProt Protein Name
Zinc finger and SCAN domain-containing protein 32
Protein Family
UniProt Gene Name
ZSCAN32
UniProt Synonym Gene Names
ZNF434; HCCS-5
UniProt Entry Name
ZSC32_HUMAN
Uniprot Description
ZNF434: May be involved in transcriptional regulation. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: nucleus
Molecular Function: protein binding; DNA binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter
Similar Products
Product Notes
The ZSCAN32 zscan32 (Catalog #AAA9212596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 159-187. The ZNF434 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF434 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the ZSCAN32 zscan32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF434, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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