Human Kallikrein 3 (KLK3) ELISA Kit | KLK3 elisa kit

Human Kallikrein 3 (KLK3) ELISA Kit

Cat. Num. AAA451683

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Synonyms: Kallikrein 3 (KLK3); Human Kallikrein 3 (KLK3) ELISA Kit; APS; KLK2A1; KLK2-A1; PSA; HK3; KLKB1; Prostate Specific Antigen; Kallikrein-Related Peptidase 3;; KLK3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of KLK3. No significant cross-reactivity or interference between KLK3 and analogues was observed.
• Sequence Length: 238

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.953-125ng/mL
• Sensitivity: 0.7ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLK3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLK3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK3 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KLK3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of KLK3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NM_001030047.1
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 25,116 Da
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• Protein Family: Glandular kallikrein
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.