Human IRAK4 (Interleukin-1 receptor-associated kinase 4) ELISA Kit | IRAK4 elisa kit

Human IRAK4 (Interleukin-1 receptor-associated kinase 4) ELISA Kit

Cat. Num. AAA7612351

• Gene Names: IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
• Reactivity: Human
• Synonyms: IRAK4 (Interleukin-1 receptor-associated kinase 4); Human IRAK4 (Interleukin-1 receptor-associated kinase 4) ELISA Kit; Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64; IRAK4; IRAK4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IRAK4. No significant cross-reactivity or interference between IRAK4 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRAK4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 20219010
• NCBI GeneID: 51135
• UniProt Accession #: Q9NWZ3; Q69FE1; Q8TDF7; Q9Y589
• Molecular Weight: Predicted: 51 kDa; ; Observed: 51 kDa
• NCBI Official Full Name: interleukin-1 receptor associated kinase 4
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 4
• NCBI Official Symbol: IRAK4
• NCBI Official Synonym Symbols: IPD1; REN64; IRAK-4; NY-REN-64
• NCBI Protein Information: interleukin-1 receptor-associated kinase 4; renal carcinoma antigen NY-REN-64
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 4
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK4
• UniProt Synonym Gene Names: IRAK-4
• UniProt Entry Name: IRAK4_HUMAN

NCBI Description

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: extracellular space; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of smooth muscle cell proliferation; cytokine and chemokine mediated signaling pathway; cytokine production; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency