Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) ELISA Kit | INa elisa kit

Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) ELISA Kit

Cat. Num. AAA2022176

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Human
• Synonyms: Internexin Neuronal Intermediate Filament Protein Alpha (INa); Human Internexin Neuronal Intermediate Filament Protein Alpha (INa) ELISA Kit; NEF5; NF-66; 66 kDa neurofilament protein; Neurofilament-66; Neurofilament 5; INa elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Internexin Neuronal Intermediate Filament Protein Alpha (INa).; No significant cross-reactivity or interference between Internexin Neuronal Intermediate Filament Protein Alpha (INa) and analogues was observed.
• Sequence Length: 499

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Internexin Neuronal Intermediate Filament Protein Alpha (INa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Internexin Neuronal Intermediate Filament Protein Alpha (INa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Internexin Neuronal Intermediate Filament Protein Alpha (INa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Internexin Neuronal Intermediate Filament Protein Alpha (INa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Internexin Neuronal Intermediate Filament Protein Alpha (INa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Internexin Neuronal Intermediate Filament Protein Alpha (INa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for INa elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66; Neurofilament-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation