Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interleukin enhancer-binding factor 2 (ILF2/NF45/PRO3063) Standard Curve.)
Human Interleukin Enhancer-Binding Factor 2 (ILF2/NF45/PRO3063) ELISA Kit | ILF2 elisa kit
Human Interleukin Enhancer-Binding Factor 2 (ILF2/NF45/PRO3063) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interleukin enhancer-binding factor 2 (ILF2/NF45/PRO3063) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Interleukin enhancer-binding factor 2 (ILF2/NF45/PRO3063) Standard Curve.)
Background: NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of this gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression. The NF45 gene encodes a 405-amino acid polypeptide with distant similarity to DNA topoisomerase II and a prokaryotic RNA polymerase that melts DNA to initiate transcription. NF45 and NF90 were expressed in several cell types, including both unstimulated and stimulated Jurkat T cells.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Uniprot Description
ILF2: Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
Protein type: RNA-binding; Transcription, coactivator/corepressor; Nucleolus
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus
Molecular Function: transferase activity; protein binding; DNA binding; double-stranded RNA binding; ATP binding
Biological Process: transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; immune response
Research Articles on ILF2
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Product Notes
The Human ILF2 ilf2 (Catalog #AAA9716041) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716041 ELISA Kit recognizes Human ILF2. It is sometimes possible for the material contained within the vial of "Interleukin Enhancer-Binding Factor 2 (ILF2/NF45/PRO3063), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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