Human Interleukin 3 (IL-3) ELISA Kit | IL-3 elisa kit

Human Interleukin 3 (IL-3) ELISA Kit

Cat. Num. AAA2800410

• Gene Names: IL3; IL-3; MCGF; MULTI-CSF
• Reactivity: Human
• Synonyms: Interleukin 3 (IL-3); Human Interleukin 3 (IL-3) ELISA Kit; IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; P-cell stimulating factor; hematopoietic growth factor; interleukin 3; mast-cell growth factor; multilineage-colony-stimulating factor; IL-3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human IL3. No significant cross-reactivity or interference between Human IL3 and analogues was observed.
• Sequence Length: 152

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for IL-3 elisa kit

This assay employs a two-site sandwich ELISA to quantitate IL3 in samples. An antibody specific for IL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 28416915
• NCBI GeneID: 3562
• NCBI Accession #: NP_000579.2
• NCBI GenBank Nucleotide #: NM_000588.3
• UniProt Accession #: P08700; Q6GS87
• Molecular Weight: 17,233 Da
• NCBI Official Full Name: interleukin-3
• NCBI Official Synonym Full Names: interleukin 3
• NCBI Official Symbol: IL3
• NCBI Official Synonym Symbols: IL-3; MCGF; MULTI-CSF
• NCBI Protein Information: interleukin-3
• UniProt Protein Name: Interleukin-3
• UniProt Gene Name: IL3
• UniProt Synonym Gene Names: IL-3; MCGF

NCBI Description

The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.