Human Interferon Kappa (IFNk) ELISA Kit | IFNk elisa kit

Human Interferon Kappa (IFNk)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2709686

• Gene Names: IFNK; IFNT1; INFE1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Interferon Kappa (IFNk); Human Interferon Kappa (IFNk)(Sandwich ELISA) ELISA Kit; ELISA Kit for Interferon Kappa (IFNk); IFNk/Interferon Kappa; IFN-K; IFNk elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Interferon Kappa (IFNk); This assay has high sensitivity and excellent specificity for detection of Interferon Kappa (IFNk). No significant cross-reactivity or interference between Interferon Kappa (IFNk) and analogues was observed.

• Applicable Applications for IFNk elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Method: Double-antibody Sandwich
• Detection Range: 7.8-500pg/mL
• Sensitivity: 3.1pg/mL
• Assay Length: 3 h
• Sample Size: 100ul (Please refer to the manual for the specific data.)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Kappa (IFNk) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Kappa (IFNk) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1 (GPX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione Peroxidase 1 (GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1 (GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glutathione Peroxidase 1 (GPX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for IFNk elisa kit

Enzyme & Kinase; Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 112789564
• NCBI GeneID: 56832
• NCBI Accession #: NP_064509.2
• NCBI GenBank Nucleotide #: NM_020124.2
• UniProt Accession #: Q9P0W0; Q5T166
• Molecular Weight: 25,218 Da
• NCBI Official Full Name: interferon kappa
• NCBI Official Synonym Full Names: interferon kappa
• NCBI Official Symbol: IFNK
• NCBI Official Synonym Symbols: IFNT1; INFE1
• NCBI Protein Information: interferon kappa
• UniProt Protein Name: Interferon kappa
• Protein Family: Interferon
• UniProt Gene Name: IFNK
• UniProt Synonym Gene Names: IFN-kappa

NCBI Description

This gene encodes a member of the type I interferon family. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. This protein is expressed in keratinocytes and the gene is found on chromosome 9, adjacent to the type I interferon cluster. [provided by RefSeq, Jul 2008]

Uniprot Description

May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.