Human Integrin Beta 4 (ITGb4) ELISA Kit | ITGb4 elisa kit

Human Integrin Beta 4 (ITGb4) ELISA Kit

Cat. Num. AAA451290

• Gene Names: ITGB4; CD104; GP150
• Reactivity: Human
• Synonyms: Integrin Beta 4 (ITGb4); Human Integrin Beta 4 (ITGb4) ELISA Kit; LY9; CD229; SLAMF3; Hly9; Mly9; ITGb4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ITGb4. No significant cross-reactivity or interference between ITGb4 and analogues was observed.
• Sequence Length: 1822

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGb4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGb4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGb4 elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ITGb4 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITGb4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITGb4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITGb4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ITGb4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 54607035
• NCBI GeneID: 3691
• NCBI Accession #: NP_000204.3
• NCBI GenBank Nucleotide #: NM_000213.4
• UniProt Accession #: P16144; O14690; O14691; O15339; O15340; O15341; Q0VF97; Q9UIQ4; A0AVL6
• Molecular Weight: 106,779 Da
• NCBI Official Full Name: integrin beta-4 isoform 1
• NCBI Official Synonym Full Names: integrin subunit beta 4
• NCBI Official Symbol: ITGB4
• NCBI Official Synonym Symbols: CD104; GP150
• NCBI Protein Information: integrin beta-4
• UniProt Protein Name: Integrin beta-4
• Protein Family: Integrin
• UniProt Gene Name: ITGB4

NCBI Description

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Integrin alpha-6/beta-4 is a receptor for laminin. Plays a critical structural role in the hemidesmosome of epithelial cells. Is required for the regulation of keratinocyte polarity and motility. ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).