Human Insulin (INS) ELISA Kit | INS elisa kit

Human Insulin (INS) ELISA Kit

Cat. Num. AAA454788

• Gene Names: INS; IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• Reactivity: Human
• Synonyms: Insulin (INS); Human Insulin (INS) ELISA Kit; INS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ILK-1. No significant cross-reactivity or interference between ILK-1 and analogues was observed.
• Sequence Length: 110

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 33.5pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ILK-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ILK-1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for INS elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ILK-1 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ILK-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ILK-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ILK-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ILK-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557671
• NCBI GeneID: 3630
• NCBI Accession #: NP_000198.1
• NCBI GenBank Nucleotide #: NM_000207.2
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 21,537 Da
• NCBI Official Full Name: insulin preproprotein
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: IDDM; ILPR; IRDN; IDDM1; IDDM2; MODY10
• NCBI Protein Information: insulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.