Human IL-36gamma ELISA Kit | IL-36gamma elisa kit

IL-36gamma ELISA Kit

Cat. Num. AAA490342

• Gene Names: IL36G; IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• Reactivity: Human
• Synonyms: IL-36gamma; IL-36gamma ELISA Kit; IL-36gamma elisa kit

• Reactivity: Human
• Specificity: This ELISA is specific for the measurement of natural and recombinant human IL-33. It does not cross-react with mouse IL-33, human IL-6, human IL-24, human IL-23, human IL-17A, human ST2, human adiponectin, mouse adiponectin, rat adiponectin, human RBP4, human resistin, human ANGPTL3, human vaspin, human Nampt, human progranulin, human FTO, human CTRP5, human ACE2, human clusterin, human leptin, human Sirtuin1.
• Sequence Length: 134

• Samples: Serum, Plasma And Cell Culture Supernatant
• Assay Type: Sandwich
• Detection Range: 0.016 ng/ml-1 ng/ml
• Sensitivity: 5 pg/ml
• Intra-assay Precision: Four samples from rheumatoid arthritis (RA) individuals of known concentrations of human IL-33 were assayed in replicates 3 times to test precision within an assay
• Inter-assay Precision: Four samples from RA individuals of known concentrations of human IL-33 were assayed in 6 separate assays to test precision between assays.
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for IL-36gamma elisa kit

Intended Uses: The IL-33 (human) ELISA Kit is to be used for the measurement of human IL-33 in serum, plasma and cell culture supernatant. This ELISA kit is for research use only.

Principle of the Assay: This assay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for the measurement of human IL-33 in biological fluids. A monoclonal antibody specific for IL-33 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, IL-33 is recognized by the addition of an affinity purified polyclonal antibody specific for IL-33 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of IL-33 in the samples!!Background/Introduction: IL-33 is a multifunctional proinflammatory cytokine. By searching databases for IL-1beta-related proteins, two cDNAs encoding mouse and human IL-33 were isolated (1). The predicted 270-amino acid human protein has a calculated molecular mass of 30 kDa. IL-33 was also discovered as a nuclear factor for the N-terminus contains a bipartite nuclear localization signal and a homeodomainlike helix-turn-helix domain with homology to Drosophila 'engrailed' and POU transcription factors (2). This protein compartmentalization and inflammatory feature resemble HMGB1 (3). IL-33 is a crucial activator of ILC2s in innate immunity and allergic inflammation. Recently it has been shown that IL-33 is inactivated by caspases (3 and 7) and activated by inflammatory proteases (4). TNF-alpha-and IL-1beta-induced expression of IL-33 was detected in primary lung, dermal, or synovial fibroblasts and keratinocytes with low levels in dendritic cells and macrophages (1, 5). The IL-33 receptor complex is comprised of ST2 (IL1RL1) and IL1R accessory protein (IL-18Rap). Engagement of the receptor complex with IL-33 recruited Myd88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1/ERK2, p38, and JNK. Addition of IL-33 to polarized mouse Th2 lymphocytes expressing ST2 resulted in increased production of Il5 and Il13, thereby stimulating allergy reaction (1) whereas IL-33 reduced the level of IFN-gamma produced by polarized mouse Th1 cells. It has been demonstrated that synovial membranes from rheumatoid arthritis (RA) patients expressed both IL-33 and ST2 in the lining layer and the interstitial sublining layers (5). Two murine RA models showed that interaction between IL-33 and ST2 plays a key role in initiation and/or worsening the disease severity via mast cells expressing ST2 (5, 7). While none of healthy individuals showed detectable IL-33 levels in serum the patients group undergoing atopic anaphylaxis exhibited a remarkable IL-33 levels (8). IL-33 and ST2 are expressed in cardiomyocytes. It has been shown that their interaction blunts the apoptosis of cardiomyocytes (9).

NCBI and Uniprot Product Information

• NCBI GI #: 514388113
• NCBI GeneID: 56300
• NCBI Accession #: NP_001265497.1
• NCBI GenBank Nucleotide #: NM_001278568.1
• NCBI Official Full Name: interleukin-36 gamma isoform 2
• NCBI Official Synonym Full Names: interleukin 36 gamma
• NCBI Official Symbol: IL36G
• NCBI Official Synonym Symbols: IL1E; IL1F9; IL1H1; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• NCBI Protein Information: interleukin-36 gamma

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]