Human IFNL4/Interferon lambda-4 ELISA Kit | IFNL4 elisa kit

Human IFNL4/Interferon lambda-4 ELISA Kit

Cat. Num. AAA2890589

• Gene Names: IFNL4; IFNAN
• Reactivity: Human
• Synonyms: IFNL4/Interferon lambda-4; Human IFNL4/Interferon lambda-4 ELISA Kit; Interferon lambda-4; IFNL4 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Interferon lambda-4,IFN-lambda-4
• Sequence Length: 179

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 78-5000 pg/mL
• Sensitivity: <39 pg/mL
• Intra-assay Precision: Intra-Assay CV: <=6.5%
• Inter-assay Precision: Inter-Assay CV: <=9.3%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IFNL4 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 482513660
• NCBI GeneID: 101180976
• NCBI Accession #: NP_001263183.2
• NCBI GenBank Nucleotide #: NM_001276254.2
• UniProt Accession #: K9M1U5
• NCBI Official Full Name: interferon lambda-4
• NCBI Official Synonym Full Names: interferon lambda 4 (gene/pseudogene)
• NCBI Official Symbol: IFNL4
• NCBI Official Synonym Symbols: IFNAN
• NCBI Protein Information: interferon lambda-4
• UniProt Protein Name: Interferon lambda-4
• Protein Family: Interferon
• UniProt Gene Name: IFNL4
• UniProt Synonym Gene Names: IFN-lambda-4
• UniProt Entry Name: IFNL4_HUMAN

NCBI Description

This gene is a polymorphic pseudogene which, in some humans, encodes the interferon (IFN) lambda 4 protein. Humans are polymorphic for the dinucleotide TT/deltaG allele. Compared to the ancestral state in non-human primates, the TT allele produces a frameshift in the coding region of this gene which is predicted to induce nonsense-mediated mRNA decay. This allele, and an allele in the first intron of this gene, have experienced a rapid increase in frequency and show indications of positive selection. The ancestral states of these alleles are associated with an impaired ability to clear hepatitis C virus. This gene, like other type III interferons (IFNs), interacts with the IFN lambda receptor complex (IFNLR) whose signaling is generally restricted to epithelial cells. This gene resides in a cluster of four type III IFN genes and at least two pseudogenes on chromosome 19q13.2. In general, interferons are produced in response to viral infection and block viral replication and propagation to uninfected cells by activating the JAK-STAT pathway and up-regulating antiviral genes. Multiple alternatively spliced transcripts have been described for this gene but their biological validity and protein coding status is still being ascertained. [provided by RefSeq, May 2017]

Uniprot Description

IFNL4: Cytokine that may trigger an antiviral response activating the JAK-STAT pathway and up-regulating specifically some interferon-stimulated genes. Belongs to the lambda interferon family. Up-regulated by polyinosinic:polycytidylic acid (polyI:C) which mimics double-stranded hepatitis C virus. 4 isoforms of the human protein are produced by alternative splicing

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity

Biological Process: defense response to virus; innate immune response; regulation of T cell activation; tyrosine phosphorylation of Stat1 protein; tyrosine phosphorylation of Stat2 protein