Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) ELISA Kit | HVCN1 elisa kit

Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) ELISA Kit

Cat. Num. AAA2020472

• Gene Names: HVCN1; HV1; VSOP
• Reactivity: Human
• Synonyms: Hydrogen Voltage Gated Channel Protein 1 (HVCN1); Human Hydrogen Voltage Gated Channel Protein 1 (HVCN1) ELISA Kit; Hv1; VSOP; Voltage Sensor Domain-Only Protein; Hydrogen voltage-gated channel 1; HVCN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Hydrogen Voltage Gated Channel Protein 1 (HVCN1).; No significant cross-reactivity or interference between Hydrogen Voltage Gated Channel Protein 1 (HVCN1) and analogues was observed.
• Sequence Length: 273

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydrogen Voltage Gated Channel Protein 1 (HVCN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydrogen Voltage Gated Channel Protein 1 (HVCN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HVCN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydrogen Voltage Gated Channel Protein 1 (HVCN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydrogen Voltage Gated Channel Protein 1 (HVCN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydrogen Voltage Gated Channel Protein 1 (HVCN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydrogen Voltage Gated Channel Protein 1 (HVCN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HVCN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 34783432
• NCBI GeneID: 84329
• Molecular Weight: 29,415 Da
• NCBI Official Full Name: HVCN1 protein
• NCBI Official Synonym Full Names: hydrogen voltage gated channel 1
• NCBI Official Symbol: HVCN1
• NCBI Official Synonym Symbols: HV1; VSOP
• NCBI Protein Information: voltage-gated hydrogen channel 1
• UniProt Protein Name: Voltage-gated hydrogen channel 1
• Protein Family: Voltage-gated hydrogen channel
• UniProt Gene Name: HVCN1
• UniProt Synonym Gene Names: VSOP; HV1
• UniProt Entry Name: HVCN1_HUMAN

NCBI Description

This gene encodes a voltage-gated protein channel protein expressed more highly in certain cells of the immune system. Phagocytic cells produce superoxide anions which require this channel protein, and in B cells this same process facilitates antibody production. This same channel protein, however, can also regulate functions in other cells including spermatozoa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

HVCN1: Mediates the voltage-dependent proton permeability of excitable membranes. Forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. Belongs to the hydrogen channel family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Channel, cation

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: voltage-gated cation channel activity; voltage-gated proton channel activity

Biological Process: sperm-egg recognition; proton transport; response to zinc ion; single fertilization; multicellular organism reproduction; response to pH