Typical Testing Data/Standard Curve (for reference only)
Human HO-1 ELISA Kit
HO-1 StressXpress ELISA Kit
Reactivity
Human
Synonyms
HO-1; HO-1 StressXpress ELISA Kit; Heme oxygenase 1; Hemox; HMOX1; HO1; Hsp32; HO-1 ELISA; Enzyme-Linked Immunosorbent Assay; HO-1 elisa kit
Reactivity
Human
Specificity
This assay is specific for HO-1. The HO-1 ELISA has been validated for the detection of human HO-1.
Detection Range
50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/ mL, 3.125 ng/mL, 1.563 ng/mL, 0.781ng/mL, and 0 ng/mL
Sensitivity
0.21 ng/mL
Preparation and Storage
4 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HO-1 elisa kit
Background/Introduction: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the to be one of the major defense mechanisms of oxidative stress (4).
Product Categories/Family for HO-1 elisa kit
References
1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.
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Product Notes
The Human HO-1 (Catalog #AAA806879) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA806879 ELISA Kit recognizes Human HO-1. It is sometimes possible for the material contained within the vial of "HO-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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