HO-1 Recombinant Protein | Hmox1 recombinant protein

Recombinant Rat HO-1

Cat. Num. AAA806934

• Gene Names: Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• Reactivity: Rat
• Applications: Western Blot
• Purity: This protein is >90% pure as determined by SDS-PAGE analysis.
• Synonyms: HO-1; Recombinant Rat HO-1; Heme-oxygenase1; Hemox; HMOX1; HO1; HO 1; Hsp32; Hmox1 recombinant protein

• Host: Rat
• Reactivity: Rat
• Purity/Purification: This protein is >90% pure as determined by SDS-PAGE analysis.
• Form/Format: Affinity Purified
• Concentration: 1.2mg/mL (varies by lot)
• Sequence Length: 289

• Applicable Applications for Hmox1 recombinant protein: Western Blot (WB) Control
• Certificate of Analysis: This product has been certified >90% pure using SDSPAGE analysis.
• Conjugate Tag: His tag
• Expression System: E Coli
• Storage Buffer: 50mM Tris/HCl pH 7.5, 5mM Bme, 0.15M NaCl, 10% glycerol
• Preparation and Storage: -20 degree C

SDS-Page

(SDS-PAGE of ~32 kDa rat HO-1 protein .)

Related Product Information for Hmox1 recombinant protein

Scientific Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs (2).There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

Product Categories/Family for Hmox1 recombinant protein

Chaperones; Heat Shock; Oxidative Stress

References

1. Froh M. et al. (2007) World J. Gastroentereol 13(25): 3478-86. 2. Elbirt K.K. and Bonkovsky H.L. (1999) Proc Assoc Am Physicians 111(5): 348-47. 3. Maines M.D., Trakshel G.M., and Kutty R.K. (1986) J Biol Chem 261: 411-419. 4. Brydun A., et al. (2007) Hypertens Res 30(4): 341-8. 5. Poss K.D. and Tonegawa S. (1997). Proc Natl Acad Sci U S A. 94: 10925-10930. 6. Yet S.F., et al. (2003) FASEB J. 17: 1759-1761. 7. Yachie A., et al. (1999) J Clin Invest. 103: 129-135.

NCBI and Uniprot Product Information

• NCBI GI #: 6981032
• NCBI GeneID: 24451
• NCBI Accession #: NP_036712.1
• NCBI GenBank Nucleotide #: NM_012580.2
• UniProt Accession #: P06762; Q5BK87
• Molecular Weight: ~32kDa
• NCBI Official Full Name: heme oxygenase 1
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 1
• NCBI Official Symbol: Hmox1
• NCBI Official Synonym Symbols: Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32
• NCBI Protein Information: heme oxygenase 1; heme oxygenase (decyclizing) 1
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: Hmox1
• UniProt Synonym Gene Names: HO-1
• UniProt Entry Name: HMOX1_RAT