Human Histone Deacetylase 1 (HDAC1) ELISA Kit | HDAC1 elisa kit

Human Histone Deacetylase 1 (HDAC1) ELISA Kit

Cat. Num. AAA2704280

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Human
• Synonyms: Histone Deacetylase 1 (HDAC1); Human Histone Deacetylase 1 (HDAC1) ELISA Kit; HDAC1; GON10; HD1; RPD3; RPD3L1; HDAC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Histone Deacetylase 1 (HDAC1).; No significant cross-reactivity or interference between Histone Deacetylase 1 (HDAC1) and analogues was observed.
• Sequence Length: 482

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Deacetylase 1 (HDAC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HDAC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Deacetylase 1 (HDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Deacetylase 1 (HDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Deacetylase 1 (HDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histone Deacetylase 1 (HDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13128860
• NCBI GeneID: 3065
• NCBI Accession #: NP_004955.2
• NCBI GenBank Nucleotide #: NM_004964.2
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 55,103 Da
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1

NCBI Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: EC 3.5.1.98; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.2-p35.1

Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; neuron projection; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; perinuclear region of cytoplasm; protein complex; Sin3 complex

Molecular Function: activating transcription factor binding; deacetylase activity; DNA binding transcription factor activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; p53 binding; protein binding; protein complex binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; transcription regulatory region DNA binding

Biological Process: ATP-dependent chromatin remodeling; beta-catenin-TCF complex assembly; blood coagulation; cellular response to oxidative stress; cellular response to tumor necrosis factor; chromatin organization; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; methylation-dependent chromatin silencing; negative regulation of apoptosis; negative regulation of canonical Wnt signaling pathway; negative regulation of cell proliferation; negative regulation of gene expression; negative regulation of insulin secretion; negative regulation of myotube differentiation; negative regulation of neuron apoptosis; negative regulation of peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of chemokine (C-X-C motif) ligand 2 production; positive regulation of interleukin-1 production; positive regulation of oligodendrocyte differentiation; positive regulation of receptor biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of tumor necrosis factor production; positive regulation of type B pancreatic cell apoptotic process; positive regulation of tyrosine phosphorylation of STAT protein; protein deacetylation; regulation of megakaryocyte differentiation; response to amphetamine; response to caffeine; response to drug; response to hyperoxia; response to lipopolysaccharide; transcription, DNA-dependent; viral process