Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human High affinity copper uptake protein 1 (SLC31A1) Standard Curve.)
Human High Affinity Copper Uptake Protein 1 (SLC31A1) ELISA Kit | SLC31A1 elisa kit
Human High Affinity Copper Uptake Protein 1 (SLC31A1) ELISA Kit
Gene Names
SLC31A1; CTR1; COPT1
Reactivity
Human
Synonyms
High Affinity Copper Uptake Protein 1 (SLC31A1); Human High Affinity Copper Uptake Protein 1 (SLC31A1) ELISA Kit; SLC31A1; COPT1; CTR1; MGC75487; hCTR1; copper transport 1 homolog; copper transporter 1; SLC31A1 elisa kit
Reactivity
Human
Specificity
Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC31A1. No significant cross-reactivity or interference between Human SLC31A1 and analogues was observed.
Sequence Length
190
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human High affinity copper uptake protein 1 (SLC31A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human High affinity copper uptake protein 1 (SLC31A1) Standard Curve.)
Related Product Information for SLC31A1 elisa kit
Description: This Human High affinity copper uptake protein 1 (SLC31A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC31A1 in samples. An antibody specific for SLC31A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC31A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC31A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC31A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated.The deduced 190-amino acid human CTR1 protein is similar to yeast CTR1 and Arabidopsis COPT1, a copper transporter also isolated by functional complementation of yeast ctr1. All 3 predicted proteins have 3 transmembrane domains and an N terminus that is rich in methionine and serine residues; the N terminus of human CTR1 is also abundant in histidines. Northern blot analysis detected 2 major CTR1 transcripts of approximately 2 kb and 5.5 kb and a less abundant transcript of about 8.5 kb in all human organs and tissues examined.
Background: Copper is an element essential for life, but excessive copper can be toxic or even lethal to the cell. Therefore, cells have developed sophisticated ways to maintain a critical copper balance, with the intake, export, and intracellular compartmentalization or buffering of copper strictly regulated.The deduced 190-amino acid human CTR1 protein is similar to yeast CTR1 and Arabidopsis COPT1, a copper transporter also isolated by functional complementation of yeast ctr1. All 3 predicted proteins have 3 transmembrane domains and an N terminus that is rich in methionine and serine residues; the N terminus of human CTR1 is also abundant in histidines. Northern blot analysis detected 2 major CTR1 transcripts of approximately 2 kb and 5.5 kb and a less abundant transcript of about 8.5 kb in all human organs and tissues examined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
high affinity copper uptake protein 1
NCBI Official Synonym Full Names
solute carrier family 31 member 1
NCBI Official Symbol
SLC31A1
NCBI Official Synonym Symbols
CTR1; COPT1
NCBI Protein Information
high affinity copper uptake protein 1
UniProt Protein Name
High affinity copper uptake protein 1
Protein Family
UniProt Gene Name
SLC31A1
UniProt Synonym Gene Names
COPT1; CTR1; hCTR1
UniProt Entry Name
COPT1_HUMAN
NCBI Description
The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper. [provided by RefSeq, Aug 2011]
Uniprot Description
SLC31A1: High-affinity, saturable copper transporter involved in dietary copper uptake. Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
Protein type: Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass; Transporter
Chromosomal Location of Human Ortholog: 9q32
Cellular Component: recycling endosome; cell soma; integral to plasma membrane; late endosome; plasma membrane
Molecular Function: copper ion transmembrane transporter activity; copper uptake transmembrane transporter activity
Biological Process: cellular copper ion homeostasis; multidrug transport; copper ion transport; transmembrane transport
Research Articles on SLC31A1
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Product Notes
The Human SLC31A1 slc31a1 (Catalog #AAA9714269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714269 ELISA Kit recognizes Human SLC31A1. It is sometimes possible for the material contained within the vial of "High Affinity Copper Uptake Protein 1 (SLC31A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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