Rat High affinity copper uptake protein 1 ELISA Kit | Slc31a1 elisa kit

Rat High affinity copper uptake protein 1 ELISA Kit

Cat. Num. AAA760245

• Gene Names: SLC31A1; CTR1; COPT1
• Reactivity: Rat
• Synonyms: High affinity copper uptake protein 1; Rat High affinity copper uptake protein 1 ELISA Kit; Slc31a1; Slc31a1 elisa kit

• Reactivity: Rat
• Sequence Length: 190

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Slc31a1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Slc31a1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Slc31a1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Slc31a1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Slc31a1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4507015
• NCBI GeneID: 1317
• NCBI Accession #: NP_001850.1
• NCBI GenBank Nucleotide #: NM_001859.3
• Molecular Weight: 21,091 Da
• NCBI Official Full Name: high affinity copper uptake protein 1
• NCBI Official Synonym Full Names: solute carrier family 31 member 1
• NCBI Official Symbol: SLC31A1
• NCBI Official Synonym Symbols: CTR1; COPT1
• NCBI Protein Information: high affinity copper uptake protein 1
• UniProt Protein Name: High affinity copper uptake protein 1
• Protein Family: High affinity copper uptake protein
• UniProt Gene Name: SLC31A1
• UniProt Synonym Gene Names: COPT1; CTR1; hCTR1
• UniProt Entry Name: COPT1_HUMAN

NCBI Description

The protein encoded by this gene is a high-affinity copper transporter found in the cell membrane. The encoded protein functions as a homotrimer to effect the uptake of dietary copper. [provided by RefSeq, Aug 2011]

Uniprot Description

SLC31A1: High-affinity, saturable copper transporter involved in dietary copper uptake. Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: cell soma; integral to plasma membrane; late endosome; plasma membrane; recycling endosome

Molecular Function: copper ion transmembrane transporter activity; copper uptake transmembrane transporter activity

Biological Process: cellular copper ion homeostasis; copper ion transport; multidrug transport; transmembrane transport