Human heme oxygenase 2 (HO-2) ELISA Kit | HO-2 elisa kit

Human heme oxygenase 2 (HO-2) ELISA Kit

Cat. Num. AAA266121

• Gene Names: HMOX2; HO-2
• Reactivity: Human
• Synonyms: heme oxygenase 2 (HO-2); Human heme oxygenase 2 (HO-2) ELISA Kit; HO-2 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 316

• Samples: Human Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Hnrnpd Concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HO-2 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human HNRNPD monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 8051608
• NCBI GeneID: 3163
• NCBI Accession #: NP_002125.3
• NCBI GenBank Nucleotide #: NM_002134.3
• Molecular Weight: 32,837 Da
• NCBI Official Full Name: heme oxygenase 2 isoform b
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 2
• NCBI Official Symbol: HMOX2
• NCBI Official Synonym Symbols: HO-2
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Heme oxygenase 2
• UniProt Gene Name: HMOX2
• UniProt Synonym Gene Names: HO2; HO-2
• UniProt Entry Name: HMOX2_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: EC 1.14.99.3; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane

Molecular Function: protein binding; metal ion binding; heme oxygenase (decyclizing) activity

Biological Process: cellular iron ion homeostasis; heme catabolic process; porphyrin metabolic process; heme oxidation; response to hypoxia; transmembrane transport