Human Heme Oxygenase 2, Decycling (HO2) ELISA Kit | HO2 elisa kit

Human Heme Oxygenase 2, Decycling (HO2) ELISA Kit

Cat. Num. AAA2021933

• Gene Names: HMOX2; HO-2
• Reactivity: Human
• Synonyms: Heme Oxygenase 2; Decycling (HO2); Human Heme Oxygenase 2; Decycling (HO2) ELISA Kit; HMOX2; HO2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 2, Decycling (HO2).; No significant cross-reactivity or interference between Heme Oxygenase 2, Decycling (HO2) and analogues was observed.
• Sequence Length: 287

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HO2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 2, Decycling (HO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 2, Decycling (HO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 2, Decycling (HO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 2, Decycling (HO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for HO2 elisa kit

Enzyme & Kinase; Metabolic pathway; Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 8051608
• NCBI GeneID: 3163
• NCBI Accession #: NP_002125.3
• NCBI GenBank Nucleotide #: NM_002134.3
• UniProt Accession #: P30519; O60605; A8MT35; D3DUD5; I3L430
• Molecular Weight: 32,837 Da
• NCBI Official Full Name: heme oxygenase 2 isoform b
• NCBI Official Synonym Full Names: heme oxygenase 2
• NCBI Official Symbol: HMOX2
• NCBI Official Synonym Symbols: HO-2
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Heme oxygenase 2
• Protein Family: Probable inactive heme oxygenase
• UniProt Gene Name: HMOX2
• UniProt Synonym Gene Names: HO2; HO-2
• UniProt Entry Name: HMOX2_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Oxidoreductase; EC 1.14.99.3

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane

Molecular Function: protein binding; metal ion binding; heme oxygenase (decyclizing) activity

Biological Process: heme catabolic process; cellular iron ion homeostasis; porphyrin metabolic process; heme oxidation; response to hypoxia; transmembrane transport