Human GZMK ELISA Kit | GZMK elisa kit

Human GZMK (Granzyme K) ELISA Kit

Cat. Num. AAA2512767

• Gene Names: GZMK; TRYP2
• Reactivity: Human
• Synonyms: GZMK; Human GZMK (Granzyme K) ELISA Kit; GZMK elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human GZMK in samples. No significant cross-reactivity or interference between Human GZMK and analogues was observed.
• Sequence Length: 264

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GZMK were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GZMK were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GZMK elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human GZMK concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GZMK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GZMK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GZMK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GZMK. You can calculate the concentration of Human GZMK in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504235
• NCBI GeneID: 3003
• NCBI Accession #: NP_002095.1
• NCBI GenBank Nucleotide #: NM_002104.2
• UniProt Accession #: P49863; B2R563
• Molecular Weight: 28,882 Da
• NCBI Official Full Name: granzyme K
• NCBI Official Synonym Full Names: granzyme K (granzyme 3; tryptase II)
• NCBI Official Symbol: GZMK
• NCBI Official Synonym Symbols: TRYP2
• NCBI Protein Information: granzyme K; NK-Tryp-2; NK-tryptase-2; fragmentin-3; granzyme 3; granzyme K (serine protease, granzyme 3; tryptase II); granzyme-3
• UniProt Protein Name: Granzyme K
• Protein Family: Granzyme
• UniProt Gene Name: GZMK
• UniProt Synonym Gene Names: TRYP2; NK-Tryp-2
• UniProt Entry Name: GRAK_HUMAN

NCBI Description

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis