Human Granzyme K (GZMK) ELISA Kit | GZMK elisa kit

Human Granzyme K (GZMK) ELISA Kit

Cat. Num. AAA2024296

• Gene Names: GZMK; TRYP2
• Reactivity: Human
• Synonyms: Granzyme K (GZMK); Human Granzyme K (GZMK) ELISA Kit; GZM-K; TRYP2; PRSS; Serine Protease; Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2; GZMK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GZMK. No significant cross-reactivity or interference between GZMK and analogues was observed.
• Sequence Length: 264

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 12.2pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GZMK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GZMK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for GZMK elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GZMK in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GZMK. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GZMK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GZMK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GZMK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GZMK elisa kit

Enzyme & Kinase; Tumor immunity; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 4504235
• NCBI GeneID: 3003
• NCBI Accession #: NP_002095.1
• NCBI GenBank Nucleotide #: NM_002104.2
• UniProt Accession #: P49863; B2R563
• Molecular Weight: 28,882 Da
• NCBI Official Full Name: granzyme K
• NCBI Official Synonym Full Names: granzyme K (granzyme 3; tryptase II)
• NCBI Official Symbol: GZMK
• NCBI Official Synonym Symbols: TRYP2
• NCBI Protein Information: granzyme K
• UniProt Protein Name: Granzyme K
• Protein Family: Granzyme
• UniProt Gene Name: GZMK
• UniProt Synonym Gene Names: TRYP2; NK-Tryp-2
• UniProt Entry Name: GRAK_HUMAN

NCBI Description

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Uniprot Description

GZMK: this protein is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008]

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: proteolysis