Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP:AMP phosphotransferase, mitochondrial (AK3) Standard Curve.)
Human GTP:AMP Phosphotransferase, Mitochondrial (AK3) ELISA Kit | AK3 elisa kit
Human GTP:AMP Phosphotransferase, Mitochondrial (AK3) ELISA Kit
Gene Names
AK3; AK6; FIX; AK3L1; AKL3L; AKL3L1
Reactivity
Human
Synonyms
GTP:AMP Phosphotransferase; Mitochondrial (AK3); Human GTP:AMP Phosphotransferase; Mitochondrial (AK3) ELISA Kit; RP11-6J24.4; AK3L1; AK6; AKL3L; AKL3L1; FIX; adenylate kinase 3 alpha like; adenylate kinase 3 like 1; adenylate kinase 6; AK3 elisa kit
Reactivity
Human
Specificity
Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit has high sensitivity and excellent specificity for detection of Human AK3. No significant cross-reactivity or interference between Human AK3 and analogues was observed.
Sequence Length
227
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP:AMP phosphotransferase, mitochondrial (AK3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP:AMP phosphotransferase, mitochondrial (AK3) Standard Curve.)
Related Product Information for AK3 elisa kit
Description: This Human GTP:AMP phosphotransferase, mitochondrial (AK3) ELISA Kit employs a two-site sandwich ELISA to quantitate AK3 in samples. An antibody specific for AK3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AK3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AK3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AK3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 227-amino acid protein has a calculated molecular mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28 kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction.Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA.
Background: The deduced 227-amino acid protein has a calculated molecular mass of 25.6 kD. AK3 shares 57.4% amino acid identity with AK4 (AK3L1). It shares 75%, 90%, and 91% amino acid identity with bovine, rat, and mouse Ak3, respectively. Northern blot analysis detected a 3.2-kb transcript in all tissues examined. Expression was highest in heart, liver, and skeletal muscle, moderate in kidney and pancreas, and weak in brain, placenta, and lung. Transcripts of 5, 1.8, and 1.35 kb were detected in heart, liver, and pancreas. Western blot analysis detected AK3 at an apparent molecular mass of 28 kD in all tissues examined. Subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction.Human embryonic kidney cells had endogenous phosphotransferase activity, which increased following transfection of AK3 cDNA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
GTP:AMP phosphotransferase AK3, mitochondrial isoform a
NCBI Official Synonym Full Names
adenylate kinase 3
NCBI Official Symbol
AK3
NCBI Official Synonym Symbols
AK6; FIX; AK3L1; AKL3L; AKL3L1
NCBI Protein Information
GTP:AMP phosphotransferase AK3, mitochondrial
UniProt Protein Name
GTP:AMP phosphotransferase AK3, mitochondrial
Protein Family
UniProt Gene Name
AK3
UniProt Synonym Gene Names
AK 3
NCBI Description
The protein encoded by this gene is a GTP:ATP phosphotransferase that is found in the mitochondrial matrix. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Has GTP:AMP phosphotransferase and ITP:AMP phosphotransferase activities.
Research Articles on AK3
Similar Products
Product Notes
The Human AK3 ak3 (Catalog #AAA9714302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714302 ELISA Kit recognizes Human AK3. It is sometimes possible for the material contained within the vial of "GTP:AMP Phosphotransferase, Mitochondrial (AK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
