Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP-binding protein Di-Ras1 (DIRAS1) Standard Curve.)
Human GTP-Binding Protein Di-Ras1 (DIRAS1) ELISA Kit | DIRAS1 elisa kit
Human GTP-Binding Protein Di-Ras1 (DIRAS1) ELISA Kit
Gene Names
DIRAS1; RIG; GBTS1; Di-Ras1
Reactivity
Human
Synonyms
GTP-Binding Protein Di-Ras1 (DIRAS1); Human GTP-Binding Protein Di-Ras1 (DIRAS1) ELISA Kit; DIRAS1; Di-Ras1; FLJ42681; GBTS1; RIG; small GTP-binding tumor suppressor 1; DIRAS1 elisa kit
Reactivity
Human
Specificity
Human GTP-binding protein Di-Ras1 (DIRAS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human DIRAS1. No significant cross-reactivity or interference between Human DIRAS1 and analogues was observed.
Sequence Length
198
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP-binding protein Di-Ras1 (DIRAS1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human GTP-binding protein Di-Ras1 (DIRAS1) Standard Curve.)
Related Product Information for DIRAS1 elisa kit
Description: This Human GTP-binding protein Di-Ras1 (DIRAS1) ELISA Kit employs a two-site sandwich ELISA to quantitate DIRAS1 in samples. An antibody specific for DIRAS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DIRAS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIRAS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIRAS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DIRAS1 (DIRAS Family GTPase 1) is a Protein Coding gene. GO annotations related to DIRAS1 include GTP binding and mitogen-activated protein kinase binding. An important paralog of DIRAS1 is DIRAS2. Displays low GTPase activity and exists predominantly in the GTP-bound form.
Background: DIRAS1 (DIRAS Family GTPase 1) is a Protein Coding gene. GO annotations related to DIRAS1 include GTP binding and mitogen-activated protein kinase binding. An important paralog of DIRAS1 is DIRAS2. Displays low GTPase activity and exists predominantly in the GTP-bound form.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
GTP-binding protein Di-Ras1
NCBI Official Synonym Full Names
DIRAS family GTPase 1
NCBI Official Symbol
DIRAS1
NCBI Official Synonym Symbols
RIG; GBTS1; Di-Ras1
NCBI Protein Information
GTP-binding protein Di-Ras1
UniProt Protein Name
GTP-binding protein Di-Ras1
Protein Family
UniProt Gene Name
DIRAS1
UniProt Synonym Gene Names
GBTS1; RIG; Rig
UniProt Entry Name
DIRA1_HUMAN
NCBI Description
DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004]
Uniprot Description
DIRAS1: Displays low GTPase activity and exist predominantly in the GTP-bound form. Belongs to the small GTPase superfamily. Di-Ras family.
Protein type: G protein, monomeric, di-Ras; G protein; G protein, monomeric
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: plasma membrane
Molecular Function: GTPase activity; GTP binding; mitogen-activated protein kinase binding
Biological Process: positive regulation of MAP kinase activity; metabolic process; small GTPase mediated signal transduction
Research Articles on DIRAS1
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Product Notes
The Human DIRAS1 diras1 (Catalog #AAA9715717) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715717 ELISA Kit recognizes Human DIRAS1. It is sometimes possible for the material contained within the vial of "GTP-Binding Protein Di-Ras1 (DIRAS1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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