Human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit | GAS6 elisa kit

Human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit

Cat. Num. AAA2021234

• Gene Names: GAS6; AXSF; AXLLG
• Reactivity: Human
• Synonyms: Growth Arrest Specific Protein 6 (GAS6); Human Growth Arrest Specific Protein 6 (GAS6) ELISA Kit; AXLLG; AXSF; AXL Stimulatory Factor; AXL receptor tyrosine kinase ligand; GAS6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Arrest Specific Protein 6 (GAS6).; No significant cross-reactivity or interference between Growth Arrest Specific Protein 6 (GAS6) and analogues was observed.
• Sequence Length: 678

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 31.2-2000pg/mL
• Sensitivity: < 12.9pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Arrest Specific Protein 6 (GAS6) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Arrest Specific Protein 6 (GAS6) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAS6 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest Specific Protein 6 (GAS6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest Specific Protein 6 (GAS6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest Specific Protein 6 (GAS6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest Specific Protein 6 (GAS6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GAS6 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 4557617
• NCBI GeneID: 2621
• NCBI Accession #: NP_000811.1
• NCBI GenBank Nucleotide #: NM_000820.3
• UniProt Accession #: Q14393; Q6IMN1; Q7Z7N3; B3KRQ7; B3KVL4; E9PBL7
• Molecular Weight: 41,986 Da
• NCBI Official Full Name: growth arrest-specific protein 6
• NCBI Official Synonym Full Names: growth arrest-specific 6
• NCBI Official Symbol: GAS6
• NCBI Official Synonym Symbols: AXSF; AXLLG
• NCBI Protein Information: growth arrest-specific protein 6
• UniProt Protein Name: Growth arrest-specific protein 6
• Protein Family: Growth arrest-specific protein
• UniProt Gene Name: GAS6
• UniProt Synonym Gene Names: AXLLG; GAS-6
• UniProt Entry Name: GAS6_HUMAN

NCBI Description

This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]

Uniprot Description

GAS6: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; cytoplasm; extracellular region

Molecular Function: voltage-gated calcium channel activity; protein binding; phosphatidylserine binding; caspase inhibitor activity; protein tyrosine kinase activator activity; calcium ion binding; receptor tyrosine kinase binding; receptor agonist activity; molecular adaptor activity; receptor binding

Biological Process: proteolysis; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; viral envelope fusion with host membrane; negative regulation of protein import into nucleus, translocation; regulation of growth; positive regulation of phagocytosis; negative regulation of interleukin-6 production; cell cycle arrest; cell adhesion; positive regulation of TOR signaling pathway; platelet activation; organ regeneration; activation of protein kinase B; negative regulation of transcription factor activity; cellular response to starvation; peptidyl-glutamic acid carboxylation; negative regulation of transcription, DNA-dependent; negative regulation of interleukin-1 secretion; leukocyte migration; negative regulation of apoptosis; entry of virus into host cell; neuron migration; negative regulation of caspase activity; signal transduction; positive regulation of natural killer cell differentiation; positive regulation of protein export from nucleus; platelet degranulation; positive regulation of glomerular filtration; negative regulation of interferon-gamma production; protein kinase B signaling cascade; virion attachment, binding of host cell surface receptor; cell migration; negative regulation of tumor necrosis factor production; viral genome replication; post-translational protein modification; positive regulation of peptidyl-serine phosphorylation; phagocytosis; cell-substrate adhesion; positive regulation of protein kinase B signaling cascade; cell proliferation; peptidyl-serine phosphorylation; cellular protein metabolic process; positive regulation of protein kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of cytokine and chemokine mediated signaling pathway; blood coagulation; apoptotic cell clearance