Human Granzyme H (GZMH) ELISA Kit | GZMH elisa kit

Human Granzyme H (GZMH) ELISA Kit

Cat. Num. AAA450294

• Gene Names: GZMH; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• Reactivity: Human
• Synonyms: Granzyme H (GZMH); Human Granzyme H (GZMH) ELISA Kit; CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2; Cathepsin G-Like 2; Protein h-CCPX; Cytotoxic T-lymphocyte proteinase; Cytotoxic serine protease C; GZMH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GZMH. No significant cross-reactivity or interference between GZMH and analogues was observed.
• Sequence Length: 160

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100pg/mL
• Sensitivity: 0.57pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GZMH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GZMH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GZMH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GZMH in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GZMH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GZMH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GZMH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GZMH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 399124767
• NCBI GeneID: 2999
• NCBI Accession #: NP_001257710.1
• NCBI GenBank Nucleotide #: NM_001270781.1
• UniProt Accession #: P20718; Q6XGZ0; Q6XGZ1; G3V2C5
• Molecular Weight: 12,609 Da
• NCBI Official Full Name: granzyme H isoform 3
• NCBI Official Synonym Full Names: granzyme H
• NCBI Official Symbol: GZMH
• NCBI Official Synonym Symbols: CCP-X; CGL-2; CSP-C; CTLA1; CTSGL2
• NCBI Protein Information: granzyme H
• UniProt Protein Name: Granzyme H
• Protein Family: Granzyme
• UniProt Gene Name: GZMH
• UniProt Synonym Gene Names: CGL2; CTSGL2; CTSGL2; CSP-C

NCBI Description

This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14. [provided by RefSeq, Nov 2015]

Uniprot Description

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.