Typical Testing Data/Standard Curve (for reference only)
Human GPER1 ELISA Kit | GPER1 elisa kit
Human GPER1 (G Protein Coupled Estrogen Receptor 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Human GM1-Ab. Standards or samples are added to the micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for Human GM1-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GM1-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GM1-Ab. You can calculate the concentration of Human GM1-Ab in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
GPER1: Receptor for estrogen. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 7p22.3
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cytoplasmic vesicle membrane; endoplasmic reticulum; integral to plasma membrane; early endosome; postsynaptic density; dendrite; nuclear envelope; trans-Golgi network; Golgi membrane; presynaptic membrane; recycling endosome; perinuclear region of cytoplasm; axon; mitochondrial membrane; cytoplasm; keratin filament; plasma membrane; presynaptic active zone; dendritic shaft; nerve terminal; intracellular; cell junction; nucleus
Molecular Function: G-protein coupled receptor activity; protein binding; estrogen receptor activity; mineralocorticoid receptor activity; chromatin binding; steroid binding
Biological Process: positive regulation of apoptosis; generation of action potential; nuclear fragmentation during apoptosis; positive regulation of caspase activity; positive regulation of vasodilation; negative regulation of DNA metabolic process; positive regulation of neurotransmitter secretion; negative regulation of leukocyte activation; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; positive regulation of MAPKKK cascade; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; inflammatory response; steroid hormone receptor signaling pathway; positive regulation of neurogenesis; cytosolic calcium ion homeostasis; negative regulation of lipid biosynthetic process; positive regulation of insulin secretion; positive regulation of inositol trisphosphate biosynthetic process; apoptotic chromosome condensation; negative regulation of fat cell differentiation; cell cycle; positive regulation of phosphoinositide 3-kinase cascade; G-protein coupled receptor protein signaling pathway; mineralocorticoid receptor signaling pathway; negative regulation of inflammatory response; positive regulation of G-protein coupled receptor protein signaling pathway; innate immune response; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of protein amino acid phosphorylation; positive regulation of cell migration
Research Articles on GPER1
Similar Products
Product Notes
The Human GPER1 gper1 (Catalog #AAA2511109) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2511109 ELISA Kit recognizes Human GPER1. It is sometimes possible for the material contained within the vial of "GPER1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
