Typical Testing Data/Standard Curve (for reference only)
Human Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) ELISA Kit | CRIM1 elisa kit
Human Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) ELISA Kit
Gene Names
CRIM1; S52; CRIM-1
Reactivity
Human
Synonyms
Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1); Human Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1) ELISA Kit; Cysteine-Rich Motor neuron 1; Chordin-Like; Cysteine-rich repeat-containing protein S52; CRIM1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CRAT. No significant cross-reactivity or interference between CRAT and analogues was observed.
Sequence Length
1,036
Samples
Human Tissue Homogenates, Cell Lysates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
3.12-200ng/mL
Sensitivity
1.31ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRAT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRAT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CRIM1 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRAT in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CRAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CRAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
113,738 Da
NCBI Official Full Name
cysteine rich transmembrane BMP regulator 1 (chordin-like)
NCBI Official Synonym Full Names
cysteine rich transmembrane BMP regulator 1
NCBI Official Symbol
CRIM1
NCBI Official Synonym Symbols
S52; CRIM-1
NCBI Protein Information
cysteine-rich motor neuron 1 protein
UniProt Protein Name
Cysteine-rich motor neuron 1 protein
Protein Family
UniProt Gene Name
CRIM1
UniProt Synonym Gene Names
S52; CRIM-1
NCBI Description
This gene encodes a transmembrane protein containing six cysteine-rich repeat domains and an insulin-like growth factor-binding domain. The encoded protein may play a role in tissue development though interactions with members of the transforming growth factor beta family, such as bone morphogenetic proteins. [provided by RefSeq, Nov 2010]
Uniprot Description
May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface.
Research Articles on CRIM1
Similar Products
Product Notes
The Human CRIM1 crim1 (Catalog #AAA2706674) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706674 ELISA Kit recognizes Human CRIM1. It is sometimes possible for the material contained within the vial of "Cysteine Rich Transmembrane BMP Regulator 1 (CRIM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
