Human GMCSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit | GMCSF elisa kit
Human GMCSF (Granulocyte Macrophage Colony Stimulating Factor) Pre-Coated ELISA Kit
Gene Names
CSF2; CSF; GMCSF
Reactivity
Human
Applications
ELISA
Synonyms
GMCSF (Granulocyte Macrophage Colony Stimulating Factor); Human GMCSF (Granulocyte Macrophage Colony Stimulating Factor) Pre-Coated ELISA Kit; GMCSF elisa kit
Reactivity
Human
Sequence Length
144
Applicable Applications for GMCSF elisa kit
ELISA (EIA)
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
3.9-250 pg/ml
Sensitivity
< 2.34 pg/ml.
Related Product Information for GMCSF elisa kit
This immunoassay kit allows for the in vitro quantitative determination of Human GMCSF concentrations in serum, plasma and other biological fluids.
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human GMCSF antibody was pre-coated into 96-well plates. Biotin conjugated anti-human GMCSF detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human GMCSF amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human GMCSF can be calculated using the standard curve.
Product Categories/Family for GMCSF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,295 Da
NCBI Official Full Name
granulocyte-macrophage colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 2
NCBI Official Symbol
CSF2
NCBI Official Synonym Symbols
CSF; GMCSF
NCBI Protein Information
granulocyte-macrophage colony-stimulating factor
UniProt Protein Name
Granulocyte-macrophage colony-stimulating factor
UniProt Gene Name
CSF2
UniProt Synonym Gene Names
GMCSF; GM-CSF; CSF
NCBI Description
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Research Articles on GMCSF
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Product Notes
The Human GMCSF csf2 (Catalog #AAA669151) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA669151 ELISA Kit recognizes Human GMCSF. AAA Biotech's GMCSF (Granulocyte Macrophage Colony Stimulating Factor) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the GMCSF csf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GMCSF (Granulocyte Macrophage Colony Stimulating Factor), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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