Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit | GDNF elisa kit

Human glial cell line-derived neurotrophic factor, GDNF ELISA Kit

Cat. Num. AAA703343

• Gene Names: GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF
• Reactivity: Human
• Synonyms: glial cell line-derived neurotrophic factor (GDNF); Human glial cell line-derived neurotrophic factor; GDNF ELISA Kit; Human glial cell line-derived neurotrophic factor (GDNF) ELISA kit; ATF1; ATF2; HFB1-GDNF; astrocyte-derived trophic factor; glial cell line derived neurotrophic factor; GDNF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GDNF. No significant cross-reactivity or interference between Human GDNF and analogues was observed.
• Sequence Length: 211

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: 19.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDNF elisa kit

Introduction: Glial Cell Line-derived Neurotrophic Factor (GDNF) is a recently discoved neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-superfamily. In humans, GDNF is encoded by the GDNF gene. This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschprung disease. The most prominent feature of GDNF is its ability to support the survival of dopaminergic and motorneurons. These neuronal populations die in the course of Parkinson's disease and amyotrophic lateral sclerosis (ALS) respectively. GDNF also regulates kidney development and spermatogenesis, and it affects alcohol consumption.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GDNF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for GDNF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GDNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503975
• NCBI GeneID: 2668
• NCBI Accession #: NP_000505.1
• NCBI GenBank Nucleotide #: NM_000514.3
• UniProt Accession #: P39905; O95448; O95449; O95986; Q6FH33; Q96L44; Q9UD32; Q9UD33; Q9UMV2; Q9UP67; Q9UP97; B7WPK7; AAB33493; AAB33494
• Molecular Weight: 23,720 Da
• NCBI Official Full Name: glial cell line-derived neurotrophic factor isoform 1 preproprotein
• NCBI Official Synonym Full Names: glial cell derived neurotrophic factor
• NCBI Official Symbol: GDNF
• NCBI Official Synonym Symbols: ATF1; ATF2; HSCR3; HFB1-GDNF
• NCBI Protein Information: glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor
• UniProt Protein Name: Glial cell line-derived neurotrophic factor
• Protein Family: Glial cell line-derived neurotrophic factor
• UniProt Gene Name: GDNF
• UniProt Synonym Gene Names: hGDNF; ATF
• UniProt Entry Name: GDNF_HUMAN

NCBI Description

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

Uniprot Description

Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.1 PublicationManual assertion based on experiment in:Ref.1