Human GATAT2 (Endothelial transcription factor GATA-2) ELISA Kit | GATAT2 elisa kit

Human GATAT2 (Endothelial transcription factor GATA-2) ELISA Kit

Cat. Num. AAA7606061

• Gene Names: GATA2; DCML; IMD21; NFE1B; MONOMAC
• Reactivity: Human
• Synonyms: GATAT2 (Endothelial transcription factor GATA-2); Human GATAT2 (Endothelial transcription factor GATA-2) ELISA Kit; GATAT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GATAT2. No significant cross-reactivity or interference between GATAT2 and analogues was observed.
• Sequence Length: 480

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GATAT2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 224611699
• NCBI GeneID: 2624
• NCBI Accession #: NP_001139133.1
• NCBI GenBank Nucleotide #: NM_001145661.1
• UniProt Accession #: P23769; Q53YE0; Q96BH0; Q96BH8; Q9BUJ6; D3DNB3
• Molecular Weight: 49,106 Da
• NCBI Official Full Name: endothelial transcription factor GATA-2 isoform 1
• NCBI Official Synonym Full Names: GATA binding protein 2
• NCBI Official Symbol: GATA2
• NCBI Official Synonym Symbols: DCML; IMD21; NFE1B; MONOMAC
• NCBI Protein Information: endothelial transcription factor GATA-2
• UniProt Protein Name: Endothelial transcription factor GATA-2
• UniProt Gene Name: GATA2

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

Uniprot Description

GATA2: Transcriptional activator which regulates endothelin-1 gene expression in endothelial cells. Binds to the consensus sequence 5'-AGATAG-3'. Endothelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 3q21.3

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; protein binding; transcription factor activity; transcription factor binding

Biological Process: blood coagulation; negative regulation of fat cell differentiation; negative regulation of Notch signaling pathway; positive regulation of angiogenesis; positive regulation of phagocytosis; positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Immunodeficiency 21; Lymphedema, Primary, With Myelodysplasia; Myelodysplastic Syndrome