Human Gastrotropin (FABP6) ELISA Kit | FABP6 elisa kit

Human Gastrotropin (FABP6) ELISA Kit

Cat. Num. AAA7233299

• Gene Names: FABP6; ILBP; I-15P; I-BAP; ILBP3; ILLBP; I-BABP; I-BALB
• Reactivity: Human
• Synonyms: Gastrotropin (FABP6); Human Gastrotropin (FABP6) ELISA Kit; FABP6 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000 pg/mL
• Sensitivity: 1.0PG/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for FABP6 elisa kit

Intended Uses: This FABP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FABP6. This ELISA kit for research use only!

Principle of the Assay: FABP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FABP6 antibody and an FABP6-HRP conjugate. The assay sample and buffer are incubated together with FABP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP6 concentration since FABP6 from samples and FABP6-HRP conjugate compete for the anti-FABP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP6 from the sample, fewer sites are left to bind FABP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP6 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94721243
• NCBI GeneID: 2172
• NCBI Accession #: NP_001035532.1
• NCBI GenBank Nucleotide #: NM_001040442.1
• Molecular Weight: 19,874 Da
• NCBI Official Full Name: gastrotropin isoform 1
• NCBI Official Synonym Full Names: fatty acid binding protein 6, ileal
• NCBI Official Symbol: FABP6
• NCBI Official Synonym Symbols: ILBP; I-15P; I-BAP; ILBP3; ILLBP; I-BABP; I-BALB
• NCBI Protein Information: gastrotropin; GT; intestinal 15 kDa protein; ileal lipid-binding protein; illeal lipid-binding protein; ileal bile acid binding protein
• UniProt Protein Name: Gastrotropin
• Protein Family: Gastrotropin
• UniProt Gene Name: FABP6
• UniProt Synonym Gene Names: ILBP; ILLBP; GT; ILBP; I-15P; I-BABP
• UniProt Entry Name: FABP6_HUMAN

NCBI Description

This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP6: Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 5q33.3-q34

Cellular Component: cytoplasm; cytosol

Molecular Function: transporter activity; lipid binding

Biological Process: bile acid and bile salt transport; negative regulation of cell proliferation; bile acid metabolic process; lipid metabolic process; transmembrane transport