Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gastrokine-2 (GKN2) Standard Curve.)
Human Gastrokine-2 (GKN2) ELISA Kit | GKN2 elisa kit
Human Gastrokine-2 (GKN2) ELISA Kit
Gene Names
GKN2; GDDR; TFIZ1; PRO813; BRICD1B; VLTI465
Reactivity
Human
Synonyms
Gastrokine-2 (GKN2); Human Gastrokine-2 (GKN2) ELISA Kit; GKN2; GDDR; PRO813; TFIZ1; VLTI465; blottin; down regulated in gastric cancer GDDR; down-regulated in gastric cancer GDDR; trefoil factor interactions(z) 1; GKN2 elisa kit
Reactivity
Human
Specificity
Human Gastrokine-2 (GKN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human GKN2. No significant cross-reactivity or interference between Human GKN2 and analogues was observed.
Sequence Length
184
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gastrokine-2 (GKN2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Gastrokine-2 (GKN2) Standard Curve.)
Related Product Information for GKN2 elisa kit
Description: This Human Gastrokine-2 (GKN2) ELISA Kit employs a two-site sandwich ELISA to quantitate GKN2 in samples. An antibody specific for GKN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GKN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GKN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GKN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Gastrokine-2 (GKN2) is a secretory peptide of human gastric surface mucous cells (SMCs). It forms disulfide-linked heterodimers with the trefoil factor family (TFF) peptide TFF1. Binding with TFF2 was also reported. Antral SMCs differ from those of the corpus by their TFF3 expression. GKN2 expression along the antral gland axis, to characterize the continuous regeneration of antral glands, and to investigate the interactions of GKN2 with TFF1, TFF2 and mucins.Maturation of antral SMCs occurs stepwise via trans-differentiation of TFF3 expressing progenitor cells. The TFF1-GKN2 heterodimer and TFF2 differ haracteristically by their binding to gastric mucins. This points to different physiological functions of TFF1 and TFF2, the latter maybe acting as a "link peptide" for stabilization of the gastric mucus.
Background: Gastrokine-2 (GKN2) is a secretory peptide of human gastric surface mucous cells (SMCs). It forms disulfide-linked heterodimers with the trefoil factor family (TFF) peptide TFF1. Binding with TFF2 was also reported. Antral SMCs differ from those of the corpus by their TFF3 expression. GKN2 expression along the antral gland axis, to characterize the continuous regeneration of antral glands, and to investigate the interactions of GKN2 with TFF1, TFF2 and mucins.Maturation of antral SMCs occurs stepwise via trans-differentiation of TFF3 expressing progenitor cells. The TFF1-GKN2 heterodimer and TFF2 differ haracteristically by their binding to gastric mucins. This points to different physiological functions of TFF1 and TFF2, the latter maybe acting as a "link peptide" for stabilization of the gastric mucus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
gastrokine-2
NCBI Official Synonym Full Names
gastrokine 2
NCBI Official Symbol
GKN2
NCBI Official Synonym Symbols
GDDR; TFIZ1; PRO813; BRICD1B; VLTI465
NCBI Protein Information
gastrokine-2
UniProt Protein Name
Gastrokine-2
Protein Family
UniProt Gene Name
GKN2
UniProt Synonym Gene Names
BLOT; GDDR; TFIZ1
UniProt Entry Name
GKN2_HUMAN
NCBI Description
The secretory protein encoded by this gene is produced in gastric surface mucous cells, where it can bind trefoil factor family peptide 1 or gastrokine-1. This gene may be a tumor suppressor gene, as its expression is markedly decreased in gastric cancer tissues. The encoded protein interacts with gastrokine-1 and regulates homeostasis of the gastric mucosa. [provided by RefSeq, Dec 2015]
Uniprot Description
GKN2: Interacts with TFF2. Heterodimer with TFF1; disulfide linked.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: extracellular region
Research Articles on GKN2
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Product Notes
The Human GKN2 gkn2 (Catalog #AAA9716314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716314 ELISA Kit recognizes Human GKN2. It is sometimes possible for the material contained within the vial of "Gastrokine-2 (GKN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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