Human Galectin 1 (GAL1) ELISA Kit | GAL1 elisa kit

Mini Samples Galectin 1 (GAL1) ELISA Kit

Cat. Num. AAA2709392

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Human
• Applications: ELISA
• Synonyms: Galectin 1 (GAL1); Mini Samples Galectin 1 (GAL1) ELISA Kit; LGALS1; GBP; HLBP14; Galaptin; Lectin; Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin; GAL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GAL1. No significant cross-reactivity or interference between GAL1 and analogues was observed.

• Applicable Applications for GAL1 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GAL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GAL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAL1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAL1 in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to GAL1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to GAL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GAL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding pro
• UniProt Protein Name: Galectin-1
• Protein Family: Gallinacin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Protein type: Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function: protein binding; signal transducer activity; protein homodimerization activity; lactose binding; laminin binding; galactoside binding; glycoprotein binding

Biological Process: myoblast differentiation; response to drug; regulation of apoptosis; plasma cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress