Typical Testing Data/Standard Curve (for reference only)
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Human Fructosamine-3-Kinase (FN3K) ELISA Kit | FN3K elisa kit
Human Fructosamine-3-Kinase (FN3K) ELISA Kit
Reactivity
Human
Synonyms
Fructosamine-3-Kinase (FN3K); Human Fructosamine-3-Kinase (FN3K) ELISA Kit; FN3K elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of FN3K. No significant cross-reactivity or interference between FN3K and analogues was observed.
Sequence Length
309
Samples
Human plasma, tissue homogenates, cell lysates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
< 0.119ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FN3K were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FN3K were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for FN3K elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FN3K in human plasma, tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FN3K. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FN3K. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FN3K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FN3K in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to FN3K. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to FN3K. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FN3K, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of FN3K in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for FN3K elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,171 Da
NCBI Official Full Name
fructosamine-3-kinase
NCBI Official Synonym Full Names
fructosamine 3 kinase
NCBI Official Symbol
FN3K
NCBI Protein Information
fructosamine-3-kinase
UniProt Protein Name
Fructosamine-3-kinase
Protein Family
UniProt Gene Name
FN3K
UniProt Entry Name
FN3K_HUMAN
NCBI Description
A high concentration of glucose can result in non-enzymatic oxidation of proteins by reaction of glucose and lysine residues (glycation). Proteins modified in this way, fructosamines, are less active or functional. This gene encodes an enzyme which catalyzes the phosphorylation of fructosamines which may result in deglycation. [provided by RefSeq, Feb 2012]
Uniprot Description
FN3K: May initiate a process leading to the deglycation of fructoselysine and of glycated proteins. May play a role in the phosphorylation of 1-deoxy-1-morpholinofructose (DMF), fructoselysine, fructoseglycine, fructose and glycated lysozyme. Belongs to the fructosamine kinase family.
Protein type: EC 2.7.1.-; Kinase, other
Chromosomal Location of Human Ortholog: 17q25.3
Molecular Function: fructosamine-3-kinase activity
Biological Process: fructoselysine metabolic process; epithelial cell differentiation; fructosamine metabolic process; phosphorylation
Research Articles on FN3K
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Product Notes
The Human FN3K fn3k (Catalog #AAA2021228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021228 ELISA Kit recognizes Human FN3K. It is sometimes possible for the material contained within the vial of "Fructosamine-3-Kinase (FN3K), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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