CLDN6 recombinant protein

Human CLDN6 (138-160) Protein, hFc Tag

• Purity: Purified from cell culture supernatant by affinity chromatography; The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: CLDN6; Human CLDN6 (138-160) Protein; hFc Tag; Claudin-6; Skullin; CLDN6 recombinant protein

• Host: HEK293
• Purity/Purification: Purified from cell culture supernatant by affinity chromatography; The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization.
• Sequence: CLDN6(138-160)(Trp138-Leu160) hFc(Glu99-Ala330)

• Tag: C-Human Fc Tag
• Molecular Characterization: CLDN6(Trp138-Leu160) hFc(Glu99-Ala330)
• Reconstitution: 1: Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5 minutes at room temperature to ensure the lyophilized sample to settle down at the bottom othe tube.; 2: Dissolved lyophilized protein sample in sterile water based on the recommended volume 51.81 ul; 3: After addin sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigourously pipette sample.
• Visual Inspection: Test: Lyophilzation ; Specification: Dried white poweder ; Resutl: Pass; Test: Vial label ; Specification: Label aligned and complete ; Result: Pass; Test: Bottle Sealing (Manual Check); Specification: Sealed tight ; Result: Pass
• Quantity: Test: Bradford Method ; Specification: >specified quantity ; Result: Pass
• Other Properties: Test: pH ; Specification: 7.4 +/- 0.5; Result: Pass
• Preparation and Storage: Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

SDS-PAGE

(Figure 1. Human CLDN6(138-160) Protein, hFc Tag on SDS-PAGE under reducing condition.)

Related Product Information for CLDN6 recombinant protein

Recombinant Human CLDN6 (138-160) Protein with C-terminal human Fc tag
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010]

Product Categories/Family for CLDN6 recombinant protein

ECD 1182; ECD Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 153792768
• NCBI GeneID: 9074
• NCBI Accession #: NP_067018.2
• NCBI GenBank Nucleotide #: NM_021195.4
• UniProt Accession #: P56747; B3KQP9; D3DUA5
• Molecular Weight: Molecular Weight Note: The protein has a predicted molecular mass of 28.9 kDa after removal of the signal peptide. The apparent molecular mass of CLDN6(138-160)-hFc is approximately 25-35 kDa due to glycosylation.
• NCBI Official Full Name: claudin-6
• NCBI Official Synonym Full Names: claudin 6
• NCBI Official Symbol: CLDN6
• NCBI Protein Information: claudin-6; skullin
• UniProt Protein Name: Claudin-6
• UniProt Gene Name: CLDN6
• UniProt Entry Name: CLD6_HUMAN