Human Filamin B Beta (FLNb) ELISA Kit | FLNb elisa kit

Human Filamin B Beta (FLNb) ELISA Kit

Cat. Num. AAA2020639

• Gene Names: FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• Reactivity: Human
• Synonyms: Filamin B Beta (FLNb); Human Filamin B Beta (FLNb) ELISA Kit; ABP-278; AOI; FH1; FLN1L; LRS1; SCT; TABP; TAP; Actin Binding Protein 278; Actin-binding-like protein; Thyroid autoantigen; Filamin-3; Truncated actin-binding protein; FLNb elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Filamin B Beta (FLNb).; No significant cross-reactivity or interference between Filamin B Beta (FLNb) and analogues was observed.
• Sequence Length: 2598

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 5.4pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Filamin B Beta (FLNb) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Filamin B Beta (FLNb) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLNb elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin B Beta (FLNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Filamin B Beta (FLNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Filamin B Beta (FLNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Filamin B Beta (FLNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FLNb elisa kit

Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 119585761
• NCBI GeneID: 2317
• Molecular Weight: 276,939 Da
• NCBI Official Full Name: filamin B, beta (actin binding protein 278), isoform CRA_e
• NCBI Official Synonym Full Names: filamin B, beta
• NCBI Official Symbol: FLNB
• NCBI Official Synonym Symbols: AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• NCBI Protein Information: filamin-B
• UniProt Protein Name: Filamin-B
• Protein Family: Filamin
• UniProt Gene Name: FLNB
• UniProt Synonym Gene Names: FLN1L; FLN3; TABP; TAP; FLN-B; Fh1; Truncated ABP
• UniProt Entry Name: FLNB_HUMAN

NCBI Description

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: focal adhesion; cytoplasm; stress fiber; plasma membrane; integral to membrane; cell cortex; Z disc; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; signal transduction; actin cytoskeleton organization and biogenesis; cell differentiation

Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii