Chicken Filamin B, Beta ELISA Kit | FLNB elisa kit

Chicken Filamin B, Beta ELISA Kit

Cat. Num. AAA740299

• Gene Names: FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• Reactivity: Chicken
• Synonyms: Filamin B; Beta; Chicken Filamin B; Beta ELISA Kit; FLNB elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This FLNB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken FLNB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FLNB elisa kit

Principle of the Assay: FLNB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FLNB antibody and an FLNB-HRP conjugate. The assay sample and buffer are incubated together with FLNB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FLNB concentration since FLNB from samples and FLNB-HRP conjugate compete for the anti-FLNB antibody binding site. Since the number of sites is limited, as more sites are occupied by FLNB from the sample, fewer sites are left to bind FLNB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FLNB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FLNB elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 256222415
• NCBI GeneID: 2317
• NCBI Accession #: NP_001157791.1
• NCBI GenBank Nucleotide #: NM_001164319.1
• UniProt Accession #: O75369; Q13706; Q59EC2; Q60FE7; Q6MZJ1; Q8WXS9; Q8WXT0; B2ZZ83; B2ZZ84; B2ZZ85; C9JKE6; C9JMC4; Q68CT4; Q8WXT3
• Molecular Weight: 278,164 Da
• NCBI Official Full Name: filamin-B isoform 4
• NCBI Official Synonym Full Names: filamin B, beta
• NCBI Official Symbol: FLNB
• NCBI Official Synonym Symbols: AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• NCBI Protein Information: filamin-B; filamin-3; beta-filamin; ABP-280 homolog; filamin homolog 1; thyroid autoantigen; actin binding protein 278; actin-binding-like protein; Larsen syndrome 1 (autosomal dominant)
• UniProt Protein Name: Filamin-B
• Protein Family: Filamin
• UniProt Gene Name: FLNB
• UniProt Synonym Gene Names: FLN1L; FLN3; TABP; TAP; FLN-B; Fh1
• UniProt Entry Name: FLNB_HUMAN

NCBI Description

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: focal adhesion; cytoplasm; integral to membrane; plasma membrane; stress fiber; cell cortex; cytosol; Z disc; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; cell differentiation; actin cytoskeleton organization and biogenesis; signal transduction

Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii