Human Ficolin-2 ELISA Kit | Fcnb elisa kit

Ficolin-2, Human, ELISA Kit

Cat. Num. AAA584550

• Gene Names: Fcnb; Fcn2
• Reactivity: Human
• Synonyms: Ficolin-2; Human; ELISA Kit; P35; Ficolin L; L-ficolin; EBP-37; hucolin; Fcnb elisa kit

• Reactivity: Human
• Sequence Length: 314

• Application Notes: Application Notes: The human Ficolin-2 ELISA has been developed for the quantitative measurement of natural Ficolin-2 in plasma and serum.
• Detection Level: 16 ng/ml
• Linearity: The linearity of the assay was determined by serially diluting a sample containing 6903 ng/ml human Ficolin-2. The diluted samples were measured in the assay. The line obtained a slope of 1.11 and a correlation coefficient of 0.998.
• Recovery: Normal human blood samples (plasma), containing baseline levels of 500 ng/ml were spiked with recombinant Ficolin-2 in concentrations of 20 and 200 ng/ml. Samples with and without Ficolin-2 were incubated for 1 hour at 37 degree C. Samples were measured using the ELISA. Recovery values for Ficolin-2 ranged between 100 % and 110%.
• Standard Range: 16-1000 ng/ml
• Working Volume: 100 ul/well
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for Fcnb elisa kit

Product Description: Ficolins are a group of proteins containing both a collagen-like domain and a fibrinogen-like domain. Three forms of ficolins have been identified in humans: Ficolin-1 (M-ficolin), Ficolin-2 (L-ficolin),and Ficolin-3 (H-ficolin). Ficolin-2 is produced primarily by the liver. Whereas Ficolin-3 is produced by the liver and the lung and Ficolin-1 by monocytes, leukocytes and lung cells. Ficolin-2 recognizes distinct danger-associated molecular patterns (DAMPs) like GlcNAc-structures in Lipoteichoic Acid (LTA) and fungal 1,3-beta-D-glucan. Ficolin-2 also recognizes N-acetylated compounds such as acetylcholine. Furthermore, Ficolin-2 recognizes apoptotic cells and participates in the removal of host cells. Ficolin-2 circulates in complex with MASP-2 and can activate the lectin complement pathway. Ficolin-2 serum levels are reported with varying concentrations. Polymorphisms in the promoter of the FCN2 gene from which Ficolin-2 is derived are associated with variation in Ficolin-2 serum levels. These polymorphisms may influence the affinity of Ficolin-2 to GlcNAc-structures. Ficolin-2 deficiencies have not been reported, but low Ficolin-2 serum levels have been associated with recurrent respiratory infections in children. Interestingly, Ficolin-2 has been implicated in the unique immune challenge during pregnancy. In maternal plasma of normal pregnancies, a 4- to 5-fold increase in Ficolin-2 was detected compared to healthy non-pregnant persons. However, significantly lower Ficolin-2 maternal plasma concentrations were associated with pre-eclamptic pregnancies. Therefore, assessment of Ficolin-2 is warranted to study its regulatory role in the innate immune system. Normal human plasma contains a Ficolin-2 concentration ranging from 1 to 14 ug/ml.
Principle of the assay: The human Ficolin-2 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3½ hours.

References

1. Atkinson, A et al; L-Ficolin in children with recurrent respiratory infections. Clin Exp Immunol 2004, 138: 517 2. Schlapbach, L et al; M-ficolin in the neonatal period: Associations with need for mechanical ventilation and mortality in premature infants with necrotising enterocolitis. Mol Immunol 2009, 46: 2597

NCBI and Uniprot Product Information

• NCBI GI #: 77404440
• NCBI GeneID: 14134
• NCBI Accession #: NP_034320.1
• NCBI GenBank Nucleotide #: NM_010190.1
• Molecular Weight: 33,984 Da
• NCBI Official Full Name: ficolin-2
• NCBI Official Synonym Full Names: ficolin B
• NCBI Official Symbol: Fcnb
• NCBI Official Synonym Symbols: Fcn2
• NCBI Protein Information: ficolin-2; L-ficolin; ficolin-B; ficolin-beta; collagen/fibrinogen domain-containing protein 2
• UniProt Protein Name: Ficolin-2
• Protein Family: Ficolin
• UniProt Gene Name: Fcn2
• UniProt Synonym Gene Names: Fcnb
• UniProt Entry Name: FCN2_MOUSE