Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) Standard Curve.)
Human Fibronectin Type-III Domain-Containing Protein C4orf31 (C4orf31) ELISA Kit | C4orf31 elisa kit
Human Fibronectin Type-III Domain-Containing Protein C4orf31 (C4orf31) ELISA Kit
Gene Names
NDNF; NORD; C4orf31
Reactivity
Human
Synonyms
Fibronectin Type-III Domain-Containing Protein C4orf31 (C4orf31); Human Fibronectin Type-III Domain-Containing Protein C4orf31 (C4orf31) ELISA Kit; C4orf31; FLJ23191; hypothetical protein LOC79625; C4orf31 elisa kit
Reactivity
Human
Specificity
Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit has high sensitivity and excellent specificity for detection of Human C4orf31. No significant cross-reactivity or interference between Human C4orf31 and analogues was observed.
Sequence Length
568
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) Standard Curve.)
Related Product Information for C4orf31 elisa kit
Description: This Human Fibronectin type-III domain-containing protein C4orf31 (C4orf31) ELISA Kit employs a two-site sandwich ELISA to quantitate C4orf31 in samples. An antibody specific for C4orf31 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4orf31 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4orf31 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4orf31 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NDNF is evolutionarily well conserved, being present in invertebrate animals. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands.NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocampal neurons against excitotoxicity and amyloid beta-peptide toxicity. NDNF was exclusively expressed in the brain and spinal cord. NDNF was expressed by neurons and not by astrocytes. NDNF expression was observed in the neurons during development.NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.
Background: NDNF is evolutionarily well conserved, being present in invertebrate animals. Multiple cysteines are conserved between species and secondary structure prediction shows that NDNF is mainly composed of beta-strands.NDNF is a glycosylated, disulfide-bonded secretory protein that contains a fibronectin type III domain. NDNF promoted migration and growth and elicited neurite outgrowth of mouse hippocampal neurons in culture. NDNF also protected cultured hippocampal neurons against excitotoxicity and amyloid beta-peptide toxicity. NDNF was exclusively expressed in the brain and spinal cord. NDNF was expressed by neurons and not by astrocytes. NDNF expression was observed in the neurons during development.NDNF is a novel neurotrophic factor derived from neurons that may be useful in the treatment of neuronal degeneration diseases and nerve injuries.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein NDNF
NCBI Official Synonym Full Names
neuron derived neurotrophic factor
NCBI Official Symbol
NDNF
NCBI Official Synonym Symbols
NORD; C4orf31
NCBI Protein Information
protein NDNF
UniProt Protein Name
Protein NDNF
UniProt Gene Name
NDNF
UniProt Synonym Gene Names
C4orf31; UNQ2748/PRO6487
UniProt Entry Name
NDNF_HUMAN
Uniprot Description
NDNF: Promotes matrix assembly and cell adhesiveness. Promotes neuron migration, growth and survival as well as neurite outgrowth.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q27
Cellular Component: extracellular matrix; extracellular region
Molecular Function: heparin binding; glycosaminoglycan binding
Biological Process: extracellular matrix organization and biogenesis; neuron migration; negative regulation of neuron apoptosis; cell growth; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan
Research Articles on C4orf31
Similar Products
Product Notes
The Human C4orf31 ndnf (Catalog #AAA9715958) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715958 ELISA Kit recognizes Human C4orf31. It is sometimes possible for the material contained within the vial of "Fibronectin Type-III Domain-Containing Protein C4orf31 (C4orf31), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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